Elucidation of population stratifying markers and selective sweeps in crossbred Landlly pig population using genome-wide SNP data

Tapendra Saini; Anuj Chauhan; Sheikh Firdous Ahmad; Amit Kumar; Sakshi Vaishnav; Shivani Singh; Arnav Mehrotra; Bharat Bhushan; G K Gaur; Triveni Dutt

doi:10.1007/s00335-024-10029-4

Elucidation of population stratifying markers and selective sweeps in crossbred Landlly pig population using genome-wide SNP data

Mamm Genome. 2024 Mar 15. doi: 10.1007/s00335-024-10029-4. Online ahead of print.

Authors

Affiliations

¹ Animal Genetics Division, ICAR-Indian Veterinary Research Institute, Izatnagar, 243122, India.
² Swine Production Farm, LPM Section, ICAR-Indian Veterinary Research Institute, Izatnagar, 243122, India. anuj.chauhan@icar.gov.in.
³ Swine Production Farm, LPM Section, ICAR-Indian Veterinary Research Institute, Izatnagar, 243122, India.
⁴ ETH Zurich, Rämistrasse 101, Zurich, 8092, Switzerland.
⁵ ADG Animal Production & Breeding, ICAR, New Delhi, 110001, India.
⁶ Indian Veterinary Research Institute, Izatnagar, 243122, India.

PMID: 38485788
DOI: 10.1007/s00335-024-10029-4

Abstract

The present study was aimed at the identification of population stratifying markers from the commercial porcine SNP 60K array and elucidate the genome-wide selective sweeps in the crossbred Landlly pig population. Original genotyping data, generated on Landlly pigs, was merged in various combinations with global suid breeds that were grouped as exotic (global pig breeds excluding Indian and Chinese), Chinese (Chinese pig breeds only), and outgroup pig populations. Post quality control, the genome-wide SNPs were ranked for their stratifying power within each dataset in TRES (using three different criteria) and FIFS programs and top-ranked SNPs (0.5K, 1K, 2K, 3K, and 4K densities) were selected. PCA plots were used to assess the stratification power of low-density panels. Selective sweeps were elucidated in the Landlly population using intra- and inter-population haplotype statistics. Additionally, Tajima's D-statistics were calculated to determine the status of balancing selection in the Landlly population. PCA plots showed 0.5K marker density to effectively stratify Landlly from other pig populations. The A-score in DAPC program revealed the Delta statistic of marker selection to outperform other methods (informativeness and F_ST methods) and that 3000-marker density was suitable for stratification of Landlly animals from exotic pig populations. The results from selective sweep analysis revealed the Landlly population to be under selection for mammary (NAV2), reproductive efficiency (JMY, SERGEF, and MAP3K20), body conformation (FHIT, WNT2, ASRB, DMGDH, and BHMT), feed efficiency (CSRNP1 and ADRA1A), and immunity (U6, MYO3B, RBMS3, and FAM78B) traits. More than two methods suggested sweeps for immunity and feed efficiency traits, thus giving a strong indication for selection in this direction. The study is the first of its kind in Indian pig breeds with a comparison against global breeds. In conclusion, 500 markers were able to effectively stratify the breeds. Different traits under selective sweeps (natural or artificial selection) can be exploited for further improvement.