Longitudinal assessment of oxidative stress markers and their relationship with exposure to PM2.5 and its bound metals in healthy participants

Yuan-Ting Hsu; Ting-Yao Su; Trong-Neng Wu; Wei-Te Wu; Saou-Hsing Liou; Ching-Huang Lai; Shau-Ku Huang

doi:10.1016/j.ijheh.2024.114348

Longitudinal assessment of oxidative stress markers and their relationship with exposure to PM_2.5 and its bound metals in healthy participants

Int J Hyg Environ Health. 2024 May:258:114348. doi: 10.1016/j.ijheh.2024.114348. Epub 2024 Mar 12.

Authors

Yuan-Ting Hsu¹, Ting-Yao Su², Trong-Neng Wu³, Wei-Te Wu⁴, Saou-Hsing Liou⁵, Ching-Huang Lai⁶, Shau-Ku Huang⁷

Affiliations

¹ Department of National Institute of Environmental Health Sciences, National Health Research Institutes, Miaoli, Taiwan; National Center for Geriatrics and Welfare Research, National Health Research Institutes, Miaoli, Taiwan. Electronic address: misara116@gmail.com.
² Department of National Institute of Environmental Health Sciences, National Health Research Institutes, Miaoli, Taiwan; School of Public Health, National Defense Medical Center, Taipei, Taiwan.
³ Department of Healthcare Administration, Asia University, Taichung, Taiwan.
⁴ Department of National Institute of Environmental Health Sciences, National Health Research Institutes, Miaoli, Taiwan. Electronic address: ader.una@gmail.com.
⁵ Department of National Institute of Environmental Health Sciences, National Health Research Institutes, Miaoli, Taiwan.
⁶ School of Public Health, National Defense Medical Center, Taipei, Taiwan. Electronic address: clai4330@gmail.com.
⁷ Department of National Institute of Environmental Health Sciences, National Health Research Institutes, Miaoli, Taiwan. Electronic address: skhuang@nhri.edu.tw.

PMID: 38479164
DOI: 10.1016/j.ijheh.2024.114348

Abstract

Objective: Exposure to ambient PM_2.5 and its bound metals poses a risk to health and disease, via, in part, oxidative stress response. A variety of oxidative stress markers have been used as markers of response, but their relevance to environmental exposure remains to be established. We evaluated, longitudinally, a battery of oxidative stress markers and their relationship with the exposure of PM_2.5 and its bound metals in a panel of healthy participants.

Material and methods: Levels of residence- and personal-based ambient air PM_2.5 and its bound metals, as well as of lung function parameters, were assessed in a total of 58 questionnaire-administered healthy never smoker participants (male, 39.7%). Levels of urinary oxidative stress markers, including Nε-(hexanoyl)-lysine (HEL; an early lipid peroxidation product), 4-hydroxynonenal (4-HNE), N7-methylguanine (N7-meG), and 8-hydroxy-2-deoxyguanosine (8-OHdG), plasma antioxidants [superoxide dismutase (SOD) and glutathione peroxidase (GPx), and urinary metals were measured by ELISA, LC-MS, and ICP-MS, respectively. The results of three repeated measurements at two-month intervals were analyzed using the Generalized Estimating Equation (GEE).

Results: After adjusting for confounders, residence- and personal-based PM_2.5 levels were positively associated with HEL (β = 0.22 and 0.18) and N7-meG (β = 0.39 and 0.13). Significant correlations were observed between personal air PM_2.5-Pb and urinary Pb with HEL (β = 0.08 and 0.26). While FVC, FEV₁, FEV₁/FVC, MMF, and PEFR predicted% were normal, a negative interaction (pollutant*time, P < 0.05) was noted for PM_2.5-V, Mn, Co, Ni, Zn, As, and Pb. Additionally, a negative interaction was found for N7-meG (β = -21.35, -18.77, -23.86) and SOD (β = -26.56, -26.18, -16.48) with FEV₁, FVC, and PEFR predicted%, respectively.

Conclusion: These findings emphasize potential links between environmental exposure, internal dose, and health effects, thereby offering valuable markers for future research on metal exposure, oxidative stress, and health outcomes.

Keywords: Air pollution; HEL; N7-meG; Oxidative stress; PM(2.5).

MeSH terms

Air Pollutants* / analysis
Environmental Exposure / analysis
Healthy Volunteers
Humans
Lead / analysis
Male
Oxidative Stress
Particulate Matter / analysis
Superoxide Dismutase

Substances

Air Pollutants
Particulate Matter
Lead
Superoxide Dismutase