CD44-Based Detection of CSCs: CD44 Immunodetection by Flow Cytometry

Lornella Seeneevassen; Anissa Zaafour; Christine Varon

doi:10.1007/978-1-0716-3730-2_5

CD44-Based Detection of CSCs: CD44 Immunodetection by Flow Cytometry

Methods Mol Biol. 2024:2777:71-81. doi: 10.1007/978-1-0716-3730-2_5.

Authors

Lornella Seeneevassen^#¹, Anissa Zaafour^#¹, Christine Varon²

Affiliations

¹ INSERM U1312 BRIC Bordeaux Institute of Oncology, University of Bordeaux, Bordeaux, France.
² INSERM U1312 BRIC Bordeaux Institute of Oncology, University of Bordeaux, Bordeaux, France. christine.varon@u-bordeaux.fr.

^# Contributed equally.

PMID: 38478336
DOI: 10.1007/978-1-0716-3730-2_5

Abstract

CD44 has been described in many malignancies as a marker of cancer stem cells (CSCs). Several techniques can be used to detect these cells. Here we detail CD44 detection by flow cytometry, a precise technique allowing to determine the percentage of positive cells and the mean fluorescent intensity reflecting the CD44 expression by cells in the samples. The protocol explained here can be used to detect CD44 from cell suspensions prepared from tissues or in vitro cell cultures.

Keywords: Antibody; CD44; Cancer; Cancer stem cell; Flow cytometry; Immunofluorescence.

MeSH terms

Flow Cytometry
Hyaluronan Receptors* / metabolism
Neoplastic Stem Cells* / metabolism

Substances

Hyaluronan Receptors