Isolation of Human Adipose-Derived Stem Cells

Elizabeth Montano; Sara Al-Ghadban; Bruce A Bunnell

doi:10.1007/978-1-0716-3762-3_1

Isolation of Human Adipose-Derived Stem Cells

Methods Mol Biol. 2024:2783:3-11. doi: 10.1007/978-1-0716-3762-3_1.

Authors

Elizabeth Montano¹, Sara Al-Ghadban¹, Bruce A Bunnell²

Affiliations

¹ Department of Microbiology, Immunology and Genetics, School of Biomedical Sciences, University of North Texas Health Sciences Center, Fort Worth, TX, USA.
² Department of Microbiology, Immunology and Genetics, School of Biomedical Sciences, University of North Texas Health Sciences Center, Fort Worth, TX, USA. Bruce.Bunnell@unthsc.edu.

PMID: 38478222
DOI: 10.1007/978-1-0716-3762-3_1

Abstract

Human adipose-derived stromal/stem cells (hASCs) are a promising source of adult stem cells used in numerous applications in regenerative medicine. We present the protocols from our laboratory for isolating and expanding hASCs. The isolation of hASCs involves the enzymatic digestion of adipose tissue and subsequent culturing of the isolated cells.

Keywords: Adipose-derived stromal/stem cells (ASCs); Biopsy; Collagenase; Expansion; Human; Lipoaspirate; Mesenchymal stem cells (MSCs); Stromal vascular fraction (SVF).

MeSH terms

Adipocytes
Adipose Tissue
Adult
Cell Differentiation
Humans
Mesenchymal Stem Cells*
Regenerative Medicine
Stromal Cells