Green manure incorporation enhanced soil labile phosphorus and fruit tree growth

Yuanyu Yang; Jianwei Zhang; Xia Chang; Lunlun Chen; Yongmin Liu; Qingwei Xu; Mengjuan Wang; Haiyan Yu; Renmei Huang; Jie Zhang; Yingxiao Hu; Qijuan Hu; Xiaojun Shi; Yuting Zhang

doi:10.3389/fpls.2024.1356224

Green manure incorporation enhanced soil labile phosphorus and fruit tree growth

Front Plant Sci. 2024 Feb 26:15:1356224. doi: 10.3389/fpls.2024.1356224. eCollection 2024.

Authors

Yuanyu Yang¹, Jianwei Zhang¹, Xia Chang¹, Lunlun Chen¹, Yongmin Liu¹, Qingwei Xu¹, Mengjuan Wang¹, Haiyan Yu¹, Renmei Huang¹, Jie Zhang¹, Yingxiao Hu¹, Qijuan Hu¹, Xiaojun Shi^{1

2}, Yuting Zhang^{1

2}

Affiliations

¹ College of Resources and Environment, Southwest University, Chongqing, China.
² Interdisciplinary Research Center for Agriculture Green Development in Yangtze River Basin, Southwest University, Chongqing, China.

Abstract

Introduction: The incorporation of green manures substantially enhances the conversion of external phosphorus (P) fertilizers and soil-reserved P into forms readily available to plants. The study aims to evaluate the influence of green manure additions on soil phosphorus dynamics and citrus growth, considering different green manure species and initial soil phosphorus levels. Additionally, the research seeks to elucidate the microbiological mechanisms underlying the observed effects.

Methods: A citrus pot experiment was conducted under both P-surplus (1.50 g·P·kg^-1) and P-deficient (0.17 g·P·kg^-1) soils with incorporating legume (Leg), non-legume (Non-Leg) or no green manure residues (CK), and ¹⁸O-P labeled KH₂PO₄ (0.5 g, containing 80‰ δ¹⁸O_p) was additionally introduced to trace the turnover characteristics of chemical P fertilizer mediated by soil microorganisms.

Results and discussion: In P-surplus soil, compared with the CK treatment, the Leg treatment significantly increased soil H₂O-P_i (13.6%), NaHCO₃-P_o (8.9%), NaOH-P_i (9.5%) and NaOH-P_o (30.0%) content. It also promoted rapid turnover of P sources into H₂O-P_i and NaHCO₃-P_i pools by enhancing the phoC (576.6%) gene abundance. In contrast, the Non-Leg treatment significantly augmented soil H₂O-P_i (9.2%) and NaHCO₃-P_o (8.5%) content, facilitating the turnover of P sources into NaHCO₃-P_i pools. Under P-deficient soil conditions, compared with the CK treatment, the Leg treatment notably raised soil H₂O-P_i (150.0%), NaHCO₃-P_i (66.3%), NaHCO₃-P_o (34.8%) and NaOH-P_i (59.0%) content, contributing to the transfer of P sources into NaHCO₃-P_i and NaOH-P_i pools. This effect was achieved through elevated ALP (33.8%) and ACP (12.9%) activities and increased pqqC (48.1%), phoC (42.9%), phoD (21.7%), and bpp (27.4%) gene abundances. The Non-Leg treatment, on the other hand, led to significant increases in soil NaHCO₃-P_i (299.0%) and NaHCO₃-P_o (132.6%) content, thereby facilitating the turnover of P sources into NaHCO₃-P_i and NaOH-P_i pools, except for the phoC gene abundance. Both Leg and Non-Leg treatments significantly improved citrus growth (7.3-20.0%) and P uptake (15.4-42.1%) in P-deficient soil but yielded no substantial effects in P-surplus soil. In summary, introducing green manure crops, particularly legume green manure, emerges as a valuable approach to enhance soil P availability and foster fruit tree growth in orchard production.

Keywords: cover crop; microbial mobilization; orchard; phosphorus turnover; stable oxygen isotopes.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the National Natural Science Foundation of China (No. 31902116) and the Agriculture Research System of China - Green Manure (No. CARS-22-G-13).