Small extracellular vesicles purification and scale-up

Xinya Zheng; Hongru Ai; Kewen Qian; Guangyao Li; Shuyi Zhang; Yitan Zou; Changhai Lei; Wenyan Fu; Shi Hu

doi:10.3389/fimmu.2024.1344681

Small extracellular vesicles purification and scale-up

Front Immunol. 2024 Feb 26:15:1344681. doi: 10.3389/fimmu.2024.1344681. eCollection 2024.

Authors

Xinya Zheng^{1

2}, Hongru Ai^{1

2}, Kewen Qian¹, Guangyao Li³, Shuyi Zhang³, Yitan Zou¹, Changhai Lei³, Wenyan Fu^{4

5}, Shi Hu^{1

2}

Affiliations

¹ Department of Biomedical Engineering, College of Basic Medical Sciences, Second Military Medical University, Shanghai, China.
² School of Gongli Hospital Medical Technology, University of Shanghai for Science and Technology, Shanghai, China.
³ Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University, Shanghai, China.
⁴ Department of Assisted Reproduction, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
⁵ Fahe Life Science and Technology Inc., Shanghai, China.

Abstract

Exosomes are small extracellular vesicles (sEVs) secreted by cells. With advances in the study of sEVs, they have shown great potential in the diagnosis and treatment of disease. However, sEV therapy usually requires a certain dose and purity of sEVs to achieve the therapeutic effect, but the existing sEV purification technology exists in the form of low yield, low purity, time-consuming, complex operation and many other problems, which greatly limits the application of sEVs. Therefore, how to obtain high-purity and high-quality sEVs quickly and efficiently, and make them realize large-scale production is a major problem in current sEV research. This paper discusses how to improve the purity and yield of sEVs from the whole production process of sEVs, including the upstream cell line selection and cell culture process, to the downstream isolation and purification, quality testing and the final storage technology.

Keywords: industrialization; purification; scale-up; small extracellular vesicles; therapeutics.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Biological Transport
Cell Culture Techniques
Cell Line
Exosomes*
Extracellular Vesicles*

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This study was supported by the National Natural Science Foundation of China (grant numbers 82322055, 81773261, 31970882, 81903140, 82041012 and 92169115); the Shanghai Rising-Star Program (grant number 19QA1411400); the Shanghai Sailing Program (19YF1438600); the Shanghai Chenguang Program (grant number 17CG35); and the Shanghai Biomedical Technology Support Project (20S11906600) and the Open Project Grant from Engineering Research Center of Cell and Therapeutic Antibody, Ministry of Education, Shanghai Jiao Tong University.