Differential antiangiogenic and anticancer activities of the active metabolites of ginsenoside Rg3

Maryam Nakhjavani; Eric Smith; Kenny Yeo; Yoko Tomita; Timothy J Price; Andrea Yool; Amanda R Townsend; Jennifer E Hardingham

doi:10.1016/j.jgr.2021.05.008

Differential antiangiogenic and anticancer activities of the active metabolites of ginsenoside Rg3

J Ginseng Res. 2024 Mar;48(2):171-180. doi: 10.1016/j.jgr.2021.05.008. Epub 2021 Jun 3.

Authors

Maryam Nakhjavani¹, Eric Smith^{1

2}, Kenny Yeo^{1

2}, Yoko Tomita^{1

2

3}, Timothy J Price^{2

3}, Andrea Yool², Amanda R Townsend^{2

3}, Jennifer E Hardingham^{1

2}

Affiliations

¹ Molecular Oncology, Basil Hetzel Institute for Translational Health Research, The Queen Elizabeth Hospital, Woodville South, SA, Australia.
² Adelaide Medical School, University of Adelaide, Adelaide, SA, Australia.
³ Medical Oncology Unit, The Queen Elizabeth Hospital, Woodville South, SA, Australia.

Abstract

Background: Epimers of ginsenoside Rg3 (Rg3) have a low bioavailability and are prone to deglycosylation, which produces epimers of ginsenoside Rh2 (S-Rh2 and R-Rh2) and protopanaxadiol (S-PPD and R-PPD). The aim of this study was to compare the efficacy and potency of these molecules as anti-cancer agents.

Methods: Crystal violet staining was used to study the anti-proliferatory action of the molecules on a human epithelial breast cancer cell line, MDA-MB-231, and human umbilical vein endothelial cells (HUVEC) and compare their potency. Cell death and cell cycle were studied using flow cytometry and mode of cell death was studied using live cell imaging. Anti-angiogenic effects of the drug were studied using loop formation assay. Molecular docking showed the interaction of these molecules with vascular endothelial growth factor receptor-2 (VEGFR2) and aquaporin (AQP) water channels. VEGF bioassay was used to study the interaction of Rh2 with VEGFR2, in vitro.

Results: HUVEC was the more sensitive cell line to the anti-proliferative effects of S-Rh2, S-PPD and R-PPD. The molecules induced necroptosis/necrosis in MDA-MB-231 and apoptosis in HUVEC. S-Rh2 was the most potent inhibitor of loop formation. In silico molecular docking predicted a good binding score between Rh2 or PPD and the ATP-binding pocket of VEGFR2. VEGF bioassay showed that Rh2 was an allosteric modulator of VEGFR2. In addition, SRh2 and PPD had good binding scores with AQP1 and AQP5, both of which play roles in cell migration and proliferation.

Conclusion: The combination of these molecules might be responsible for the anti-cancer effects observed by Rg3.

Keywords: Anticancer; Epimer; Ginsenoside Rg3; Ginsenoside Rh2; Protopanaxadiol.