Dicarbonyl-Modified Low-Density Lipoproteins Are Key Inducers of LOX-1 and NOX1 Gene Expression in the Cultured Human Umbilical Vein Endotheliocytes

Biochemistry (Mosc). 2023 Dec;88(12):2125-2136. doi: 10.1134/S0006297923120143.

Abstract

Expression of LOX-1 and NOX1 genes in the human umbilical vein endotheliocytes (HUVECs) cultured in the presence of low-density lipoproteins (LDL) modified with various natural dicarbonyls was investigated for the first time. It was found that among the investigated dicarbonyl-modified LDLs (malondialdehyde (MDA)-modified LDLs, glyoxal-modified LDLs, and methylglyoxal-modified LDLs), the MDA-modified LDLs caused the greatest induction of the LOX-1 and NOX1 genes, as well as of the genes of antioxidant enzymes and genes of proapoptotic factors in HUVECs. Key role of the dicarbonyl-modified LDLs in the molecular mechanisms of vascular wall damage and endothelial dysfunction is discussed.

Keywords: dicarbonyl-modified LDL; expression of LOX-1 and NOX1 genes; low-density lipoproteins (LDL); molecular mechanisms of atherogenesis and diabetogenesis.

MeSH terms

  • Cells, Cultured
  • Endothelial Cells* / metabolism
  • Gene Expression
  • Humans
  • Lipoproteins, LDL* / metabolism
  • NADPH Oxidase 1 / genetics
  • NADPH Oxidase 1 / metabolism
  • Scavenger Receptors, Class E / genetics
  • Scavenger Receptors, Class E / metabolism
  • Umbilical Veins / metabolism

Substances

  • Lipoproteins, LDL
  • Scavenger Receptors, Class E
  • NOX1 protein, human
  • NADPH Oxidase 1