Protocol to monitor cardiac function and hemodynamics in septic rodents

Binbin Meng; Xinrun Wang; Li Li; Haisong Zhang; Wenchao Li; Zhonghua Hu; Lina Zhang; Zhaoxin Qian

doi:10.1016/j.xpro.2024.102942

Protocol to monitor cardiac function and hemodynamics in septic rodents

STAR Protoc. 2024 Mar 15;5(1):102942. doi: 10.1016/j.xpro.2024.102942. Epub 2024 Mar 7.

Authors

Binbin Meng¹, Xinrun Wang¹, Li Li¹, Haisong Zhang¹, Wenchao Li¹, Zhonghua Hu², Lina Zhang³, Zhaoxin Qian⁴

Affiliations

¹ Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Provincial Clinical Research Center for Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
² Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Provincial Clinical Research Center for Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Institute of Molecular Precision Medicine and Hunan Key Laboratory of Molecular Precision Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Key Laboratory of Animal Models for Human Diseases, School of Life Sciences, Central South University, Changsha, Hunan 410008, China. Electronic address: huzhonghua@csu.edu.cn.
³ Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Provincial Clinical Research Center for Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China. Electronic address: zln7095@csu.edu.cn.
⁴ Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Provincial Clinical Research Center for Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China. Electronic address: xyqzx@csu.edu.cn.

Abstract

Septic cardiomyopathy is associated with high mortality in septic patients, characterized by reversible systolic and diastolic dysfunction. It is essential to monitor cardiac function and hemodynamic changes in septic animals. Here, we present a protocol to monitor cardiac function and hemodynamics in septic rodents. We describe steps for performing cecal ligation and puncture on rodents to induce sepsis, acquiring two-dimensional echocardiographic and M-mode ultrasonic images, and assessing mean arterial pressure in septic animals. For complete details on the use and execution of this protocol, please refer to Zhang et al.¹.

Keywords: Immunology; Model Organisms.

MeSH terms

Animals
Echocardiography
Hemodynamics
Humans
Rodentia*
Sepsis*