Isolation and genomic characterization of S taphylococcus aureus bacteriophages from Chennai, India

Shankaregowdanakopalu Jagadeesh Deepak; Porteen Kannan; Wilfred Ruban Savariraj; Anbazhagan S; Elango Ayyasamy; Senthil Kumar Tuticorin Maragatham Alagesan; Narendra Babu Ravindran; Sureshkannan Sundaram; Nithya Quintoil Mohanadasse; Teresa D Shippy; Charley A Cull; Gizem Levent; Raghavendra G Amachawadi

doi:10.1128/mra.01209-23

Isolation and genomic characterization of S taphylococcus aureus bacteriophages from Chennai, India

Microbiol Resour Announc. 2024 Apr 11;13(4):e0120923. doi: 10.1128/mra.01209-23. Epub 2024 Mar 8.

Authors

Affiliations

¹ Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, TANUVAS, Chennai, India.
² Department of Livestock Products and Technology, Veterinary College, KVAFSU, Bengaluru, India.
³ ICMR-National Animal Resource Facility for Biomedical Research (NARFBR), Hyderabad, Telangana, India.
⁴ Veterinary College and Research Institute, TANUVAS, Salem, India.
⁵ Zoonoses Research Laboratory, Centre for Animal Health Studies, TANUVAS, Chennai, India.
⁶ Department of Livestock Products and Technology, Madras Veterinary College, TANUVAS, Chennai, India.
⁷ Department of Veterinary Public Health and Epidemiology, RIVER, Pondicherry, India.
⁸ Bioinformatics Center, Division of Biology, Kansas State University, Manhattan, Kansas, USA.
⁹ Midwest Veterinary Services, Inc., Oakland, Nebraska, USA.
¹⁰ Texas Tech University School of Veterinary Medicine, Amarillo, Texas, USA.
¹¹ Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA.

Abstract

We isolated and characterized two lytic bacteriophages against Staphylococcus aureus named TANUVAS_MVC-VPHSA1 and TANUVAS_MVC-VPHSA2, with the aim of investigating their genomic and structural features. The bacteriophages belong to the Caudoviricetes, and their genomes have sizes of 50,505 and 50,516 base pairs with a GC content of 41.4%.

Keywords: Caudoviricetes; DNA sequencing; lytic bacteriophages.

Abstract

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