Analysis of the experimental absorption spectrum of the rabbit lung and identification of its components

Maria R Pinheiro; Valery V Tuchin; Luís M Oliveira

doi:10.1002/jbio.202300494

Analysis of the experimental absorption spectrum of the rabbit lung and identification of its components

J Biophotonics. 2024 Mar 7:e202300494. doi: 10.1002/jbio.202300494. Online ahead of print.

Authors

Maria R Pinheiro^{1

2}, Valery V Tuchin^{3

4}, Luís M Oliveira^{1

5}

Affiliations

¹ Institute for Systems and Computer Engineering, Technology and Science (INESC TEC), Porto, Portugal.
² Department of Electrical and Computer Engineering, Porto University - Faculty of Engineering, Porto, Portugal.
³ Science Medical Center, Saratov State University, Saratov, Russian Federation.
⁴ Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, Tomsk, Russian Federation.
⁵ Physics Department, Polytechnic Institute of Porto - School of Engineering, Porto, Portugal.

PMID: 38453152
DOI: 10.1002/jbio.202300494

Abstract

The broadband absorption coefficient spectrum of the rabbit lung presents some particular characteristics that allow the identification of the chromophores in this tissue. By performing a weighted combination of the absorption spectra of water, hemoglobin, DNA, proteins and the pigments melanin and lipofuscin, it was possible to obtain a good match to the experimental absorption spectrum of the lung. Such reconstruction provided reasonable information about the contents of the tissue components in the lung tissue, and allowed to identify a similar accumulation of melanin and lipofuscin.

Keywords: absorption spectrum reconstruction; lung absorption spectrum; photon diffusion approximation; pigment accumulation; spectral physiological information; tissue component identification.

Abstract

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