Host specific Eimeria genus diagnosis and qPCR development in Ovis aries and Capra hircus

Shijie Li; Yichen Jian; Kaihui Zhang; Xiaoying Li; Rongjun Wang; Longxian Zhang; Fuchun Jian

doi:10.1016/j.mimet.2024.106910

Host specific Eimeria genus diagnosis and qPCR development in Ovis aries and Capra hircus

J Microbiol Methods. 2024 May:220:106910. doi: 10.1016/j.mimet.2024.106910. Epub 2024 Mar 6.

Authors

Shijie Li¹, Yichen Jian², Kaihui Zhang¹, Xiaoying Li¹, Rongjun Wang¹, Longxian Zhang¹, Fuchun Jian³

Affiliations

¹ College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
² College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
³ College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China. Electronic address: jfchun2008@163.com.

PMID: 38452905
DOI: 10.1016/j.mimet.2024.106910

Abstract

The objective of the present study was to develop a real-time PCR (qPCR) technique for the diagnosis of Eimeria spp. in Ovis aries and Capra hircus. The qPCR technique was developed using SYBR Green, resulting in a PCR with high sensitivity, specificity, and reproducibility.

Keywords: 18S rRNA; Capra hircus; Coccidiosis; Eimeria; Ovis aries; qPCR.

MeSH terms

Animals
Eimeria*
Goats
Real-Time Polymerase Chain Reaction
Reproducibility of Results
Sheep, Domestic*