Characteristics of amylose-lipid complex prepared from pullulanase-treated rice and wheat flour

Food Sci Biotechnol. 2023 Aug 28;33(5):1113-1122. doi: 10.1007/s10068-023-01411-0. eCollection 2024 Apr.

Abstract

This study aimed to evaluate the properties of amylose-lipid complexes in rice and wheat flours utilizing pullulanase as a debranching enzyme. Rice and flour were both treated with pullulanase before being combined with free fatty acids to form compounds denoted as RPF (rice-pullulanase-fatty acid) and FPF (flour-pullulanase-fatty acid), respectively. Our results showed that RPF and FPF had higher complex index and lower hydrolysis values than enzyme-untreated amylose-lipid complexes. Furthermore, RPF and FPF demonstrated lower swelling power and higher water solubility values, indicating changes in the physical properties of the starches. In vivo studies showed that RPF and FPF caused a smaller increase in blood glucose levels than untreated rice and flour, highlighting their potential use as functional food ingredients. These findings provide valuable information for the development of novel rice-and wheat-based foods with improved nutritional and physiological properties.

Supplementary information: The online version contains supplementary material available at 10.1007/s10068-023-01411-0.

Keywords: Flour; Oleic acid; Pullulanase; Resistant starch; Rice.