L-theanine attenuates H2O2-induced inflammation and apoptosis in IPEC-J2 cells via inhibiting p38 MAPK signaling pathway

Abstract

This study investigated the protective effects of L-theanine on hydrogen peroxide (H₂O₂)-induced intestinal barrier dysfunction in IPEC-J2 cells. Results showed that L-theanine reduced H₂O₂-induced IPEC-J2 cells inflammation and apoptosis, and decreased protein phosphorylation levels of p38 mitogen-activated protein kinase (p38 MAPK) and nuclear factor kappa-B (NF-κB). The p38 MAPK inhibitor (SB203580) decreased oxidative stress, the protein expression of phosphorylation of p38 MAPK and NF-κB, the H₂O₂-induced increase in mRNA expression of pro-apoptotic and pro-inflammatory related genes expression and secretion, and tight junction protein related genes expression, which was similar to the effect of L-theanine. In conclusion, L-theanine inhibited H₂O₂-induced oxidative damage and inflammatory reaction, eliminated apoptosis, and protected intestinal epithelial barrier damage by inhibiting the activation of p38 MAPK signaling pathway.

Keywords: H(2)O(2); IPEC-J2 cells; Intestinal epithelial barrier dysfunction; L-theanine; p38 MAPK.