Evaluation of gamma irradiation effects on antioxidant capacity of propolis

Ralitsa B Mladenova; Nikolay Y Solakov; Kamelia K Loginovska

doi:10.1016/j.apradiso.2024.111254

Evaluation of gamma irradiation effects on antioxidant capacity of propolis

Appl Radiat Isot. 2024 May:207:111254. doi: 10.1016/j.apradiso.2024.111254. Epub 2024 Feb 24.

Authors

Ralitsa B Mladenova¹, Nikolay Y Solakov², Kamelia K Loginovska²

Affiliations

¹ Institute of Catalysis, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bldg. 11, 1113, Sofia, Bulgaria. Electronic address: ralitsa@ic.bas.bg.
² Institute of Cryobiology and Food Technologies, Agricultural Academy, 1407, Sofia, Bulgaria.

PMID: 38430826
DOI: 10.1016/j.apradiso.2024.111254

Abstract

The irradiation effects on antioxidant potential and on content of phenolic compounds of propolis ethanoic extracts were studied. It was found out that gamma treatment of samples with 2 and 10 kGy had a weak decreasing effect on the total phenolic content (TPC), while no change was observed in the propolis irradiated with 5 kGy. The antiradical activity of extracts was assessed by the DPPH free radical scavenging activity evaluated by Electron Paramagnetic Resonance (EPR) spectroscopy. The EPR results were in agreement with TPC. Some main phenolic compounds of the studied non-irradiated and irradiated samples were identified and compared by ultra-high performance liquid chromatography (UHPLC).

Keywords: Antioxidant activity; EPR; Gamma irradiation; HPLC; Propolis; Total polyphenolics.

MeSH terms

Antioxidants*
Phenols / chemistry
Propolis* / chemistry

Substances

Antioxidants
Propolis
Phenols