Cellular senescence molecules expression in type 2 diabetes mellitus: CDKN2A, CDKN2B, and lncRNA ANRIL

Soheila Anaraki; Masoumeh Kheirandish; Pegah Mousavi; Atefe Ebrahimi Tamandegani; Samane Mohammadi; Mohammad Shekari

doi:10.1016/j.gene.2024.148319

Cellular senescence molecules expression in type 2 diabetes mellitus: CDKN2A, CDKN2B, and lncRNA ANRIL

Gene. 2024 Jun 15:911:148319. doi: 10.1016/j.gene.2024.148319. Epub 2024 Feb 28.

Authors

Soheila Anaraki¹, Masoumeh Kheirandish², Pegah Mousavi³, Atefe Ebrahimi Tamandegani¹, Samane Mohammadi¹, Mohammad Shekari⁴

Affiliations

¹ Department of Medical Genetics, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
² Endocrinology and Metabolism Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
³ Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
⁴ Department of Medical Genetics, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran. Electronic address: mohammad.sshekari@gmail.com.

PMID: 38428622
DOI: 10.1016/j.gene.2024.148319

Abstract

Aims: Cellular senescence in type 2 diabetes mellitus (T2DM) has received widespread attention. However, the cellular senescence molecules involved in T2DM are unclear. Furthermore, there are no consistent biomarkers for cellular senescence in T2DM. Therefore, this study aimed to identify cellular senescence molecules in T2DM and investigate their expression in peripheral blood mononuclear cells of individuals with T2DM.

Methods: Patients with T2DM (n = 40) and healthy controls (n = 40) were enrolled. We used different databases to identify cellular senescence molecules in T2DM and confirmed the obtained genes and lncRNA using real-time PCR.

Results: Bioinformatics analysis indicated that CDKN2A and CDKN2B genes, and long noncoding RNA ANRIL are the most effective cellular senescence molecules in T2DM. Furthermore, CDKN2A and ANRIL expression decreased in individuals with T2DM.

Conclusions: Cellular senescence may have a protective effect against T2DM. In addition, the cellular senescence molecules CDKN2A and ANRIL may be potential biomarkers of cellular senescence in T2DM.

Keywords: Biomarker; CDKN2B-AS1; Cyclin-dependent kinase inhibitor 2A/2B; Long noncoding RNA; Peripheral blood mononuclear cells; Real-time PCR.

MeSH terms

Biomarkers
Cellular Senescence / genetics
Cyclin-Dependent Kinase Inhibitor p16 / genetics
Diabetes Mellitus, Type 2* / genetics
Humans
Leukocytes, Mononuclear
RNA, Long Noncoding* / genetics

Substances

RNA, Long Noncoding
Biomarkers
CDKN2A protein, human
Cyclin-Dependent Kinase Inhibitor p16