Fabrication of diosmin loaded food-grade bilayer nanoparticles with modified chitosan and soy peptides and antioxidant properties examination

Sen Li; Tong Guan; Hongyan Lv; Yuwei Cai; Wanqing Cao; Ze Zhang; Hongdong Song; Hongwei Cao; Xiao Guan

doi:10.1016/j.fochx.2024.101237

Fabrication of diosmin loaded food-grade bilayer nanoparticles with modified chitosan and soy peptides and antioxidant properties examination

Food Chem X. 2024 Feb 18:21:101237. doi: 10.1016/j.fochx.2024.101237. eCollection 2024 Mar 30.

Authors

Sen Li^{1

2}, Tong Guan^{1

2}, Hongyan Lv^{1

2}, Yuwei Cai^{1

2}, Wanqing Cao^{1

2}, Ze Zhang^{1

2}, Hongdong Song^{1

2}, Hongwei Cao^{1

2}, Xiao Guan^{1

2}

Affiliations

¹ School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
² National Grain Industry (Urban Grain and Oil Security) Technology Innovation Center, Shanghai 200093, China.

Abstract

Diosmin is a flavonoid derived from plants, possessing anti-inflammatory, antioxidant, antidiabetic, neuroprotective and cardiovascular protective properties. However, diosmin has low solubility in water, leading to low bioavailability. In this study, we constructed bilayer nanoparticles with trimethyl chitosan and soy peptides to improve the oral bioaccessibility and bioavailability of diosmin, and determined the characteristics and antioxidant properties of the diosmin-loaded nanoparticles. The results showed that the size of the nanoparticles was around 250 nm with the encapsulation efficiency higher than 97 %, and the nanoparticles were stable under regular conditions. In vitro digestion suggested the nanoparticles could protect diosmin from releasing in gastric digestion but promote the bioaccessibility of diosmin in intestine. Furthermore, the diosmin-loaded nanoparticles presented excellent antioxidant activities in vitro and significantly decreased the Lipopolysaccharides-induced brain Malondialdehyde (MDA) level by oral administration. Therefore, the reported nanoparticles may be an effective platform for improving the oral bioavailability of diosmin.

Keywords: Antioxidant activity; Diosmin; Nanoparticles; Soy peptides; Trimethyl chitosan.