Divergent responses of phosphorus solubilizing bacteria with P-laden biochar for enhancing nutrient recovery, growth, and yield of canola (Brassica napus L.)

Abstract

The growing global population has led to a heightened need for food production, and this rise in agricultural activity is closely tied to the application of phosphorus-based fertilizers, which contributes to the depletion of rock phosphate (RP) reserves. Considering the limited P reserves, different approaches were conducted previously for P removal from waste streams, while the adsorption of ions is a novel strategy with more applicability. In this study, a comprehensive method was employed to recover phosphorus from wastewater by utilizing biochar engineered with minerals such as calcium, magnesium, and iron. Elemental analysis of the wastewater following a batch experiment indicated the efficiency of the engineered biochar as an adsorbent. Subsequently, the phosphorus-enriched biochar, hereinafter (PL-BCsb), obtained from the wastewater, underwent further analysis through FTIR, XRD, and nutritional assessments. The results revealed that the PL-BCsb contained four times higher (1.82%) P contents which further reused as a fertilizer supplementation for Brassica napus L growth. PL-BCsb showed citric acid (34.03%), Olsen solution (10.99%), and water soluble (1.74%) P desorption. Additionally, phosphorous solubilizing bacteria (PSB) were incorporated with PL-BCsb along two P fertilizer levels P45 (45 kg ha^-1) and P90 (90 kg ha^-1) for evaluation of phosphorus reuse efficiency. Integrated application of PL-BCsb with half of the suggested amount of P45 (45 kg ha^-1) and PSB increased growth, production, physiological, biochemical, and nutritional qualities of canola by almost two folds when compared to control. Similarly, it also improved soil microbial biomass carbon up to four times, alkaline and acid phosphatases activities both by one and half times respectively as compared to control P (0). Furthermore, this investigation demonstrated that waste-to-fertilizer technology enhanced the phosphorus fertilizer use efficiency by 55-60% while reducing phosphorus losses into water streams by 90%. These results have significant implications for reducing eutrophication, making it a promising approach for mitigating environmental pollution and addressing climate change.

Keywords: Phosphorus laden biochar (PL-BCsb); Phosphorus solubilizing bacteria (PSB); Sustainable phosphorus utilization; Wastewater treatment.