Generation of post-surgical minimal residual disease models to investigate metastasis in soft tissue sarcoma patient-derived orthotopic xenografts

Suzanne Fischer; David Creytens; Stefanie Gijsels; Benedicte Descamps; Lore Lapeire; An Hendrix; Gwen Sys; Olivier De Wever

doi:10.1016/j.xpro.2024.102863

Generation of post-surgical minimal residual disease models to investigate metastasis in soft tissue sarcoma patient-derived orthotopic xenografts

STAR Protoc. 2024 Mar 15;5(1):102863. doi: 10.1016/j.xpro.2024.102863. Epub 2024 Feb 28.

Authors

Suzanne Fischer¹, David Creytens², Stefanie Gijsels³, Benedicte Descamps⁴, Lore Lapeire⁵, An Hendrix⁶, Gwen Sys⁷, Olivier De Wever⁸

Affiliations

¹ Laboratory of Experimental Cancer Research, Ghent University, Ghent, Belgium; Cancer Research Institute Ghent, Ghent University, Ghent, Belgium; Department of Gastro-Intestinal Surgery, Ghent University Hospital, Ghent, Belgium. Electronic address: suzanne.fischer@ugent.be.
² Cancer Research Institute Ghent, Ghent University, Ghent, Belgium; Department of Pathology, Ghent University Hospital, Ghent, Belgium.
³ Laboratory of Experimental Cancer Research, Ghent University, Ghent, Belgium; Cancer Research Institute Ghent, Ghent University, Ghent, Belgium; Department of Gastro-Intestinal Surgery, Ghent University Hospital, Ghent, Belgium.
⁴ Animalarium, Radiological and Radiobiological Techniques, Histology Core, Ghent University, Ghent, Belgium.
⁵ Cancer Research Institute Ghent, Ghent University, Ghent, Belgium; Department of Medical Oncology, Ghent University Hospital, Ghent, Belgium.
⁶ Laboratory of Experimental Cancer Research, Ghent University, Ghent, Belgium; Cancer Research Institute Ghent, Ghent University, Ghent, Belgium.
⁷ Laboratory of Experimental Cancer Research, Ghent University, Ghent, Belgium; Cancer Research Institute Ghent, Ghent University, Ghent, Belgium; Department of Orthopedics and Traumatology, Ghent University Hospital, Ghent, Belgium.
⁸ Laboratory of Experimental Cancer Research, Ghent University, Ghent, Belgium; Cancer Research Institute Ghent, Ghent University, Ghent, Belgium. Electronic address: olivier.dewever@ugent.be.

Abstract

Despite optimal multimodal treatment including surgical resection, 50%-80% of high-grade soft tissue sarcoma (STS) patients metastasize. Here, we present a protocol for the generation and use of post-surgical minimal residual disease models to investigate metastatic relapse in STS patient-derived xenografts. We describe steps for orthotopic engraftment of high-grade STS patient-derived tumor tissue. We then detail procedures for primary tumor resection with broad, negative resection margins and follow-up until metastases using MRI. For complete details on the use and execution of this protocol, please refer to Fischer et al. (2023).¹.

Keywords: Cancer; Clinical Protocol; Model Organisms.

MeSH terms

Heterografts
Humans
Magnetic Resonance Imaging
Neoplasm, Residual
Sarcoma* / diagnostic imaging
Sarcoma* / pathology
Sarcoma* / surgery
Soft Tissue Neoplasms* / diagnostic imaging
Soft Tissue Neoplasms* / pathology
Soft Tissue Neoplasms* / surgery