Real-time OCT feedback-controlled RPE photodisruption in ex vivo porcine eyes using 8 microsecond laser pulses

Christian Burri; Simon Salzmann; Jasmin Wandel; Leonie Hoffmann; Boris Považay; Christoph Meier; Martin Frenz

doi:10.1364/BOE.503941

Real-time OCT feedback-controlled RPE photodisruption in ex vivo porcine eyes using 8 microsecond laser pulses

Biomed Opt Express. 2023 Nov 20;14(12):6328-6349. doi: 10.1364/BOE.503941. eCollection 2023 Dec 1.

Authors

Christian Burri^{1

2}, Simon Salzmann¹, Jasmin Wandel³, Leonie Hoffmann¹, Boris Považay¹, Christoph Meier¹, Martin Frenz²

Affiliations

¹ optoLab, Institute for Human Centered Engineering, Bern University of Applied Sciences, Biel, Switzerland.
² Biomedical Photonics Group, Institute of Applied Physics, University of Bern, Bern, Switzerland.
³ Institute for Optimisation and Data Analysis, Bern University of Applied Sciences, Burgdorf, Switzerland.

Abstract

Selective retinal pigment epithelium (RPE) photodisruption requires reliable real-time feedback dosimetry (RFD) to prevent unwanted overexposure. In this study, optical coherence tomography (OCT) based RFD was investigated in ex vivo porcine eyes exposed to laser pulses of 8 µs duration (wavelength: 532 nm, exposure area: 90 × 90 µm², radiant exposure: 247 to 1975 mJ/µm²). For RFD, fringe washouts in time-resolved OCT M-scans (central wavelength: 870 nm, scan rate: 85 kHz) were compared to an RPE cell viability assay. Statistical analysis revealed a moderate correlation between RPE lesion size and applied treatment energy, suggesting RFD adaptation to inter- and intraindividual RPE pigmentation and ocular transmission.