Analysis of Secondary Metabolites of Elaeagnus Angustifolia Leaves Based on UPLC-Q-TOF-MS

Jinfa Liao; Liyan Liu; Lu Yang; Wei Sheng; Ke Zhang; Bin Zhou; Silin Yu; Yongzhi Yin; Jinhui Wang

doi:10.1093/jaoacint/qsae017

Analysis of Secondary Metabolites of Elaeagnus Angustifolia Leaves Based on UPLC-Q-TOF-MS

J AOAC Int. 2024 Feb 28:qsae017. doi: 10.1093/jaoacint/qsae017. Online ahead of print.

Authors

Jinfa Liao^{1

2}, Liyan Liu², Lu Yang², Wei Sheng², Ke Zhang¹, Bin Zhou², Silin Yu^{2

3}, Yongzhi Yin^{1

2}, Jinhui Wang^{1

2

3}

Affiliations

¹ Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, College of Pharmacy, Shihezi University, Shihezi, 832002 China.
² Xinjiang Academy of Forestry, Key Laboratory of Forest Resources and Utilization in Xinjiang of National Forestry and Grassland Administration, Key Laboratory Of Fruit Tree Species Breeding And Cultivation In Xinjiang Urumqi 830052, China.
³ College of Pharmacy, Harbin Medical University, Harbin, 150081 China.

PMID: 38419590
DOI: 10.1093/jaoacint/qsae017

Abstract

Background: The leaves of Elaeagnus angustifolia, belonging to the Elaeagnaceae Juss. family, are known for their medicinal properties for relieving cough and asthma, as well as treating dysentery and diarrhea.

Objective: To establish a rapid qualitative method for the detection of secondary metabolites in leaves of Elaeagnus angustifolia, including the identification and analysis of various secondary metabolites in leaves of Elaeagnus angustifolia.

Method: Samples were separated using a Waters ACQUITY H-Class ultra-performance liquid chromatography (UPLC) system (FTN autosampler, quaternary LC pump) and ACQUITY UPLC® BEH C18 column (1.7 μm, 2.1 mm× 100 mm). The flow rate was set to 0.4 mL/min, the injection volume was 1.0 μL, and the column temperature was set to 45 °C. The mobile phase was methanol (A) with) -0.1% formic acid in water (B). Samples were analyzed by quadrupole time-of-flight mass spectrometry (Q-TOF-MS).

Results: A total of 182 different secondary metabolites were detected from 10 varieties of leaves of Elaeagnus angustifolia, including 77 flavonoids, 20 steroids, 7 alkaloids, 15 amino acids, 18 organic acids, and 45 other compound types.

Conclusions: A method for the rapid analysis of leaves of Elaeagnus angustifolia by UPLC-Q-TOF-MS was established, and the secondary metabolites in leaves of Elaeagnus angustifolia were identified. The enrichment of secondary metabolites in leaves of different varieties of Elaeagnus angustifolia was clarified.

Highlights: The UPLC-Q-TOF-MS method is very fast and possesses a high degree of selectivity, precision, and sensitivity. These findings provide a reliable foundation for the development of medicinal resources derived from Elaeagnus angustifolia leaves.