Comparison of miR-106b, miR-191 and miR-30d expression dynamics in milk with regard to its composition in Holstein and Ayrshire cows

Marina Vladimirovna Pozovnikova; Viktoria Borisovna Leibova; Olga Vasilievna Tulinova; Elena Anatolyevna Romanova; Artem Pavlovich Dysin; Natalia Viktorovna Dementieva; Anastasiia Ivanovna Azovtseva; Sergey Evgenievich Sedykh

doi:10.5713/ab.23.0427

Comparison of miR-106b, miR-191 and miR-30d expression dynamics in milk with regard to its composition in Holstein and Ayrshire cows

Anim Biosci. 2024 Feb 28. doi: 10.5713/ab.23.0427. Online ahead of print.

Authors

Marina Vladimirovna Pozovnikova¹, Viktoria Borisovna Leibova¹, Olga Vasilievna Tulinova¹, Elena Anatolyevna Romanova¹, Artem Pavlovich Dysin¹, Natalia Viktorovna Dementieva¹, Anastasiia Ivanovna Azovtseva¹, Sergey Evgenievich Sedykh²

Affiliations

¹ Russian Research Institute of Farm Animal Genetics and Breeding-Branch of the L.K. Ernst Federal Research Center for Animal Husbandry, 55A, Moskovskoye Sh., Tyarlevo, Pushkin, St. Petersburg, 196625, Russia.
² Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 8, Ac. Lavrentieva ave., Novosibirsk, 630090, Russia.

PMID: 38419530
DOI: 10.5713/ab.23.0427

Abstract

Objective: Milk composition varies considerably and depends on paratypical, genetic, and epigenetic factors. MiRNAs belong to the class of small non-coding RNAs; they are one of the key tools of epigenetic control because of their ability to regulate gene expression at the post-transcriptional level. We compared the relative expression levels of miR-106b, miR-191, and miR-30d in milk to demonstrate the relationship between the content of these miRNAs with protein and fat components of milk in Holstein and Ayrshire cattle.

Methods: Milk fat, protein, and casein contents were determined in the obtained samples, as well as the content of the main fatty acids (g/100 g milk), including: saturated acids, such as myristic (C14:0), palmitic (C16:0), and stearic (C18:0) acids; monounsaturated acids, including oleic (C18:1) acid; as well as long-, medium- and short-chain, polyunsaturated, and trans fatty acids. Real-time stem-loop one-tube reverse transcription PCR (RT-qPCR) with TaqMan probes was used to measure the miRNA expression levels.

Results: The miRNA expression levels in milk samples were found to be decreased in the first two months in Holstein breed, and in the first four months in Ayrshire breed. Correlation analysis did not reveal any dependence between changes in the expression level of miRNA and milk fat content, but showed a multidirectional relationship with individual milk fatty acids. Positive associations between the expression levels of miR-106b and miR-30d and protein and casein content were found in the Ayrshire breed. Receiver operating characteristic (ROC) curve analysis showed that miR-106b and miR-30d expression levels can cause changes in fatty acid and protein composition of milk in Ayrshire cows, whereas miR-106b expression level determines the fatty acid composition in Holsteins.

Conclusion: The data obtained in this study showed that miR-106b, miR-191, and miR-30d expression levels in milk samples have peculiarities associated with breed affiliation and the lactation period.

Keywords: Casein; Lactation; Milk Fatty Acids; Reverse Transcription.