LncRNA S100PBP promotes proliferation and steroid hormone synthesis of granulosa cells by sponging MiR-2285bc-BMPR2 in bovine

Kai Wang; Ying Cheng; Yongping Ren; Haoyu Xiu; Wenxiang Meng; Tong Guo; Xiaoyan Ma; Ermias Kebreab; Dong Wang; Lihua Lyu

doi:10.1093/biolre/ioae033

LncRNA S100PBP promotes proliferation and steroid hormone synthesis of granulosa cells by sponging MiR-2285bc-BMPR2 in bovine

Biol Reprod. 2024 Feb 27:ioae033. doi: 10.1093/biolre/ioae033. Online ahead of print.

Authors

Kai Wang¹, Ying Cheng¹, Yongping Ren¹, Haoyu Xiu¹, Wenxiang Meng¹, Tong Guo², Xiaoyan Ma¹, Ermias Kebreab³, Dong Wang⁴, Lihua Lyu¹

Affiliations

¹ College of Animal Science, Shanxi Agricultural University, Taigu, Shanxi, China, 030801.
² Department of Animal Husbandry and Veterinary Medicine, Beijing Vocational College of Agriculture, Beijing 102442, China.
³ Department of Animal Science, University of California Davis Davis, CA, 95616.
⁴ Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China, 100193.

PMID: 38412119
DOI: 10.1093/biolre/ioae033

Abstract

In bovine follicular development, the proliferation of bovine granulosa cells (GCs) affect follicular selection, atresia, and cystic follicle formation. With the proliferation of GCs and secretion of steroid hormones, follicles develop further and increase in diameter. However, when cystic follicles appear on the ovaries, GCs stop proliferating, resulting in the reduction of GCs layer. In our previous study, the whole transcriptome sequencing revealed that Bone morphogenetic protein receptor 2 (BMPR2) was differentially expressed (DE) between cystic and normal follicular GCs. We speculated that lncRNA may act as ceRNA targeting miRNAs and then regulating the expression of BMPR2 and the function of GCs, thereby affecting follicular development and cyst formation. In this study, the results elucidated that lncRNA S100PBP (NONBTAT011846.2) directly bound miR-2285bc, which targeted in the BMPR2 3'-UTR. miR-2285bc suppresses GCs proliferation by downregulating BMPR2 expression. Furthermore, lncRNA S100PBP was silenced by small interfering RNA (siRNA), and lncRNA S100PBP regulated BMPR2 expression by sponging miR-2285bc investigated through cross-verification. When siRNA of lncRNA S100PBP was transfected into GCs, the results revealed similar molecular changes as those transfected with miR-2285bc mimics. Silencing lncRNA S100PBP or overexpressing miR-2285bc altered the expressions of some follicular development-related genes, which could be related to follicular cyst occurrence. In conclusion, our findings support that lncRNA S100PBP regulates the expression of BMPR2 through sponge miR-2285bc, promotes the proliferation of GCs, inhibits their apoptosis, and increases the synthesis and secretion of follicular steroid hormones, thus promoting the development of bovine follicles and potentially inhibiting the formation of follicular cysts.

Keywords: BMPR2; bovine; follicular cyst; granulosa cells; miR-2285bc; proliferation.