In this book chapter, we present a method for microsome isolation from the hypocotyl tissue of dark-grown Arabidopsis thaliana. Microsomes are heterogeneous, vesicle-like membranes, which are, not exclusively, derived but enriched with membranes of the endoplasmic reticulum (ER). Here, we describe the experimental setup, including sample preparation, homogenization, differential centrifugation steps, and quality control measures after microsome isolation.
Keywords: Arabidopsis thaliana; Dark-grown hypocotyl; Endoplasmic reticulum (ER); Membrane-bound protein; Microsomes; Ultracentrifugation.
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.