Automated Segmentation and 3D Reconstruction of Different Membranes from Confocal Z-Stacks

Abstract

Confocal laser scanning microscopy (CLSM) is an advanced microscopy technique based on fluorescence technology which produces sharp images of a specimen in a single focal plane. The optical sectioning by CLSM allows to have z-stacks which can be further processed into 3D reconstructions. These then provide the option of variable perspectives and additional precise data evaluation on structural and anatomical alterations. Here, we used CLSM to image the thylakoids of cyanobacteria and the endoplasmic reticulum (ER) in moss protonemata as an example. Then, out of the confocal z-stacks, we create 3D constructions of the membranes and their alterations to present a holistic, structural view from different angles.

Keywords: 3D reconstruction; Amira; CLSM; Cyanobacteria; Cyanophages; DNA; Moss protonemata; Thylakoids.