Green and inventive fluorescence approach for levodropropizine determination in human plasma

Abstract

A green, rapid and sensitive fluorimetric method to quantify levodropropizine (LVP) in human plasma was exploited for the first time. The proposed method adopts LVP's intrinsic fluorescence in distilled water at a detecting emission of 345 nm following excitation at 240 nm. LVP displayed linearity across concentrations ranging from 50 to 1000 ng mL^-1, with a detection limit of 0.77 ng mL^-1 and a quantification limit of 2.33 ng mL^-1. Thorough validation confirmed its reliability, successfully determining LVP in tablets with an average recovery of 98.64 ± 1.07 %. Furthermore, the method's applicability extended to estimate the studied drug in spiked human plasma with excellent obtained percentage recoveries (98.68 ± 1.28-100.14 ± 1.23).

Keywords: Fluorimetric; Intrinsic fluorescence; Levodropropizine; Spiked Human Plasma; Tablets.