Dual function of magnetic nanocomposites-based SERS lateral flow strip for simultaneous detection of aflatoxin B1 and zearalenone

Limei Yin; Jianrong Cai; Lixin Ma; Tianyan You; Muhammad Arslan; Heera Jayan; Xiaobo Zou; Yunyun Gong

doi:10.1016/j.foodchem.2024.138817

Dual function of magnetic nanocomposites-based SERS lateral flow strip for simultaneous detection of aflatoxin B1 and zearalenone

Food Chem. 2024 Jul 15:446:138817. doi: 10.1016/j.foodchem.2024.138817. Epub 2024 Feb 20.

Authors

Limei Yin¹, Jianrong Cai², Lixin Ma², Tianyan You³, Muhammad Arslan², Heera Jayan², Xiaobo Zou², Yunyun Gong⁴

Affiliations

¹ International Joint Research Laboratory of Intelligent Agriculture and Agri-products Processing, School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China; China Light Industry Key Laboratory of Food Intelligent Detection & Processing, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: yinlm6@163.com.
² China Light Industry Key Laboratory of Food Intelligent Detection & Processing, School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
³ International Joint Research Laboratory of Intelligent Agriculture and Agri-products Processing, School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China.
⁴ School of Food Science and Nutrition, University of Leeds, Leeds LS2 9JT, UK. Electronic address: Y.Gong@leeds.ac.uk.

PMID: 38401299
DOI: 10.1016/j.foodchem.2024.138817

Abstract

Aflatoxin B1 (AFB1) and zearalenone (ZEN) are two mycotoxins that often co-occur in corn. A surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-LFIA) that can simultaneously detect AFB1 and ZEN in corn samples was developed employing the core-interlayer-satellite magnetic nanocomposites (Fe₃O₄@PEI/Au^MBA@Ag^MBA) as dual-functional SERS tags. Under the optimal conditions, the detection ranges of AFB1 and ZEN in corn samples were 0.1-10 μg/kg and 4-400 μg/kg, respectively. Moreover, the test results for two mycotoxins in contaminated corn samples employing the suggested SERS-LFIA was in line with those of the HPLC technique. In view of its satisfactory sensitivity, accuracy, precision and short testing time (20 min), the developed system has a promising application prospect in the on-site simultaneous detection of AFB1 and ZEN.

Keywords: Aflatoxin B1; Fe(3)O(4); Lateral flow; Magnetic; Surface-enhanced Raman scattering; Zearalenone.

MeSH terms

Aflatoxin B1 / analysis
Limit of Detection
Magnetic Phenomena
Magnetics
Mycotoxins* / analysis
Zea mays
Zearalenone* / analysis

Substances

Zearalenone
Aflatoxin B1
Mycotoxins