Copper regulation of PtRhRuCu nanozyme targeted boosting peroxidase-like activity for ultrasensitive smartphone-assisted colorimetric sensing of glucose

Xinpeng Zhi; Qi Yang; Xinghao Zhang; Hanbo Zhang; Ya Gao; Lulu Zhang; Yuping Tong; Weiwei He

doi:10.1016/j.foodchem.2024.138788

Copper regulation of PtRhRuCu nanozyme targeted boosting peroxidase-like activity for ultrasensitive smartphone-assisted colorimetric sensing of glucose

Food Chem. 2024 Jul 1:445:138788. doi: 10.1016/j.foodchem.2024.138788. Epub 2024 Feb 17.

Authors

Xinpeng Zhi¹, Qi Yang², Xinghao Zhang³, Hanbo Zhang³, Ya Gao³, Lulu Zhang³, Yuping Tong⁴, Weiwei He⁵

Affiliations

¹ School of Civil Engineering and Communication, North China University of Water Resources and Electric Power, Zhengzhou, Henan 450045, PR China; Key Laboratory of Micro-Nano Materials for Energy Storage and Conversion of Henan Province, Institute of Surface Micro and Nano Materials, College of Chemical and Materials Engineering, Xuchang University, Xuchang, Henan 461000, PR China.
² Key Laboratory of Micro-Nano Materials for Energy Storage and Conversion of Henan Province, Institute of Surface Micro and Nano Materials, College of Chemical and Materials Engineering, Xuchang University, Xuchang, Henan 461000, PR China. Electronic address: yangq@xcu.edu.cn.
³ Key Laboratory of Micro-Nano Materials for Energy Storage and Conversion of Henan Province, Institute of Surface Micro and Nano Materials, College of Chemical and Materials Engineering, Xuchang University, Xuchang, Henan 461000, PR China.
⁴ School of Civil Engineering and Communication, North China University of Water Resources and Electric Power, Zhengzhou, Henan 450045, PR China. Electronic address: yptong_zz@163.com.
⁵ Key Laboratory of Micro-Nano Materials for Energy Storage and Conversion of Henan Province, Institute of Surface Micro and Nano Materials, College of Chemical and Materials Engineering, Xuchang University, Xuchang, Henan 461000, PR China. Electronic address: heweiweixcu@gmail.com.

PMID: 38394910
DOI: 10.1016/j.foodchem.2024.138788

Abstract

Point-of-care testing (POCT) is promising for biodetection in home healthcare due to advantages of simplicity, rapidity, low cost, portability, high sensitivity and accuracy, and object-oriented POCT platform can be developed by nanozyme-based biosensing. However, designing high-performance nanozymes with targeted regulated catalytic activity remains challenging. Herein, advanced PtRhRuCu quaternary alloy nanozymes (QANs) were rationally designed and successfully synthesized. Cu atoms induced mechanisms of hydrogen peroxide (H₂O₂) activation and d-band center regulation, achieving high enhancement of peroxide (POD)-like activity and inhibition of oxidase (OXD)-like activity. Inspired by this, a smartphone-assisted colorimetric platform integrated with test strips was established for glucose detection of soft drinks, with a detection limit of 0.021 mM and a recovery rate of 97.87 to 103.36 %. This work not only provides a novel path for tuning specific enzyme-like activities of metal nanozymes, but also shows the potential feasibility for rational design of POCT sensors in actual samples.

Keywords: Colorimetric sensing; Glucose detection; Nanozyme; Peroxidase-like activity; Quaternary alloy; Smartphone-assisted.

MeSH terms

Colorimetry
Copper*
Glucose*
Hydrogen Peroxide
Peroxidases
Smartphone

Substances

Copper
Glucose
Hydrogen Peroxide
Peroxidases