Unraveling the Dynamics of SARS-CoV-2 Mutations: Insights from Surface Plasmon Resonance Biosensor Kinetics

Devi Taufiq Nurrohman; Nan-Fu Chiu

doi:10.3390/bios14020099

Unraveling the Dynamics of SARS-CoV-2 Mutations: Insights from Surface Plasmon Resonance Biosensor Kinetics

Biosensors (Basel). 2024 Feb 13;14(2):99. doi: 10.3390/bios14020099.

Authors

Devi Taufiq Nurrohman¹, Nan-Fu Chiu^{1

2}

Affiliations

¹ Laboratory of Nano-Photonics and Biosensors, Institute of Electro-Optical Engineering, National Taiwan Normal University, Taipei 11677, Taiwan.
² Department of Life Science, National Taiwan Normal University, Taipei 11677, Taiwan.

Abstract

Surface Plasmon Resonance (SPR) technology is known to be a powerful tool for studying biomolecular interactions because it offers real-time and label-free multiparameter analysis with high sensitivity. This article summarizes the results that have been obtained from the use of SPR technology in studying the dynamics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutations. This paper will begin by introducing the working principle of SPR and the kinetic parameters of the sensorgram, which include the association rate constant (k_a), dissociation rate constant (k_d), equilibrium association constant (K_A), and equilibrium dissociation constant (K_D). At the end of the paper, we will summarize the kinetic data on the interaction between angiotensin-converting enzyme 2 (ACE2) and SARS-CoV-2 obtained from the results of SPR signal analysis. ACE2 is a material that mediates virus entry. Therefore, understanding the kinetic changes between ACE2 and SARS-CoV-2 caused by the mutation will provide beneficial information for drug discovery, vaccine development, and other therapeutic purposes.

Keywords: COVID-19; biosensors; kinetics; surface plasmon resonance.

Publication types

Review

MeSH terms

Angiotensin-Converting Enzyme 2
Biosensing Techniques* / methods
COVID-19*
Humans
Mutation
Protein Binding
SARS-CoV-2 / genetics
Surface Plasmon Resonance / methods

Substances

Angiotensin-Converting Enzyme 2

Grants and funding

This study was funded by the Ministry of Science and Technology of the Republic of China (ROC), Taiwan, for financially supporting this research under Contract No. MOST 108-2221-E-003-020-MY3, MOST 109-2221-E-003-028-MY3. NSTC 112-2221-E-003-016-MY3. This work is sponsored by “Higher Education Sprout Project” of National Taiwan Normal University and the Ministry of Education (MOE) in Taiwan.