3D bioprinting cowpea mosaic virus as an immunotherapy depot for ovarian cancer prevention in a preclinical mouse model

Zhongchao Zhao; Yi Xiang; Edward C Koellhoffer; Sourabh Shukla; Steven Fiering; Shaochen Chen; Nicole F Steinmetz

doi:10.1039/d3ma00899a

3D bioprinting cowpea mosaic virus as an immunotherapy depot for ovarian cancer prevention in a preclinical mouse model

Mater Adv. 2024 Feb 1;5(4):1480-1486. doi: 10.1039/d3ma00899a. eCollection 2024 Feb 19.

Authors

Zhongchao Zhao^{1

2

3}, Yi Xiang¹, Edward C Koellhoffer⁴, Sourabh Shukla¹, Steven Fiering^{5

6}, Shaochen Chen^{1

2

7

8}, Nicole F Steinmetz^{1

2

3

4

7

8

9

10}

Affiliations

¹ Department of NanoEngineering, University of California San Diego, 9500 Gilman Dr. La Jolla CA, 92093 USA chen168@ucsd.edu nsteinmetz@ucsd.edu.
² Center for Nano-ImmunoEngineering, University of California San Diego, 9500 Gilman Dr. La Jolla CA 92093 USA.
³ Moores Cancer Center, University of California San Diego, 9500 Gilman Dr. La Jolla CA 92093 USA.
⁴ Department of Radiology, University of California San Diego, 9500 Gilman Dr. La Jolla CA 92093 USA.
⁵ Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth Lebanon NH 03756 USA.
⁶ Dartmouth Cancer Center, Geisel School of Medicine at Dartmouth Lebanon NH 03756 USA.
⁷ Department of Bioengineering, University of California San Diego, 9500 Gilman Dr. La Jolla CA 92093 USA.
⁸ Institute for Materials Discovery and Design, University of California San Diego, 9500 Gilman Dr. La Jolla CA 92093 USA.
⁹ Center for Engineering in Cancer, University of California San Diego, 9500 Gilman Dr. La Jolla CA 92093 USA.
¹⁰ Shu and K.C. Chien and Peter Farrell Collaboratory, University of California San Diego, 9500 Gilman Dr. La Jolla CA 92093 USA.

Abstract

Implantable polymeric hydrogels loaded with immunostimulatory cowpea mosaic virus (CPMV) were fabricated using digital light processing (DLP) printing technology. The CPMV-laden hydrogels were surgically implanted into the peritoneal cavity to serve as depots for cancer slow-release immunotherapy. Sustained release of CPMV within the intraperitoneal space alleviates the need for repeated dosing and we demonstrated efficacy against ovarian cancer in a metastatic mouse model.