Explorative study for the rapid detection of Fritillaria using gas chromatography-ion mobility spectrometry

Yuping Dai; Shanshuo Liu; Li Yang; Ye He; Xiao Guo; Yang Ma; Shunxiang Li; Dan Huang

doi:10.3389/fnut.2024.1361668

Explorative study for the rapid detection of Fritillaria using gas chromatography-ion mobility spectrometry

Front Nutr. 2024 Feb 6:11:1361668. doi: 10.3389/fnut.2024.1361668. eCollection 2024.

Authors

Yuping Dai^#^{1

2

3}, Shanshuo Liu^#¹, Li Yang⁴, Ye He¹, Xiao Guo¹, Yang Ma¹, Shunxiang Li^{1

3

5}, Dan Huang^{1

3

5}

Affiliations

¹ State Key Laboratory of Chinese Medicine Powder and Medicine Innovation in Hunan (Incubation), Science and Technology Innovation Center, Hunan University of Chinese Medicine, Changsha, China.
² Hunan Fenghuang Lanke Traditional Chinese Medicine Co., Ltd., Changsha, China.
³ Hunan Engineering Technology Research Center for Bioactive Substance Dis-covery of Chinese Medicine, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
⁴ Chongqing Healn Drug Sales Co., Ltd., Chongqing, China.
⁵ Hunan Province Sino-US International Joint Research Center for Therapeutic Drugs of Senile Degenerative Diseases, Changsha, China.

^# Contributed equally.

Abstract

Fritillaria is a well-known health-promoting food, but it has many varieties and its market circulation is chaotic. In order to explore the differences in volatile organic compounds (VOCs) among different varieties of Fritillaria and quickly and accurately determine the variety of Fritillaria, this study selected six varieties of Fritillaria and identified and analyzed their volatile components using gas chromatography-ion mobility spectrometry (GC-IMS), establishing the characteristic fingerprints of VOCs in Fritillaria. In all samples, a total of 76 peaks were detected and 67 VOCs were identified. It was found that the composition of VOCs in different varieties of Fritillaria was similar, but the content was different. Combined with chemometric analysis, the differences between VOCs were clearly shown after principal component analysis, cluster analysis, and partial least-squares discriminant analysis. This may provide theoretical guidance for the identification and authenticity determination of different varieties of Fritillaria.

Keywords: CA; Fritillaria; PCA; PLS-DA; gas chromatography-ion mobility spectrometry.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was funded by the Special Development Project of Technical Innovation and Application in Chongqing (No. CSTC2021jscx-gksb-N0031), Key Discipline Project on Chinese Pharmacology of Hunan University of Chinese Medicine (202302), Department of Science and Technology of Xiangxi Tujiazu & Miaozu Autonomous Prefecture (No. 2022JSGG03), and Department of Science and Technology of Hunan Province (No. 2021CB1012). This study received funding from Chongqing Healn Drug Sales Co., Ltd. and Hunan Fenghuang Lanke Traditional Chinese Medicine Co., Ltd. The funders have the following involvement with the conceptualization, methodology, investigation, data curation, formal analysis, and writing – original draft and editing.