In situ single-cell profiling sheds light on IFI27 localisation during SARS-CoV-2 infection

Chin Wee Tan; Jinjin Chen; Ning Liu; Dharmesh D Bhuva; Tony Blick; James Monkman; Caroline Cooper; Malvika Kharbanda; Kristen Feher; Belinda Phipson; Emily E Killingbeck; Liuliu Pan; Youngmi Kim; Yan Liang; Andy Nam; Michael Leon; Paulo Souza-Fonseca-Guimaraes; Seigo Nagashima; Ana Paula Camargo Martins; Cleber Machado-Souza; Lucia de Noronha; Benjamin Tang; Kirsty Short; John Fraser; Gabrielle T Belz; Fernando Souza-Fonseca-Guimaraes; Arutha Kulasinghe; Melissa J Davis

doi:10.1016/j.ebiom.2024.105016

In situ single-cell profiling sheds light on IFI27 localisation during SARS-CoV-2 infection

EBioMedicine. 2024 Mar:101:105016. doi: 10.1016/j.ebiom.2024.105016. Epub 2024 Feb 19.

Authors

Chin Wee Tan¹, Jinjin Chen², Ning Liu³, Dharmesh D Bhuva³, Tony Blick⁴, James Monkman⁴, Caroline Cooper⁵, Malvika Kharbanda³, Kristen Feher³, Belinda Phipson², Emily E Killingbeck⁶, Liuliu Pan⁶, Youngmi Kim⁶, Yan Liang⁶, Andy Nam⁶, Michael Leon⁶, Paulo Souza-Fonseca-Guimaraes⁷, Seigo Nagashima⁸, Ana Paula Camargo Martins⁸, Cleber Machado-Souza⁹, Lucia de Noronha⁸, Benjamin Tang¹⁰, Kirsty Short¹¹, John Fraser¹², Gabrielle T Belz⁴, Fernando Souza-Fonseca-Guimaraes⁴, Arutha Kulasinghe¹³, Melissa J Davis¹⁴

Affiliations

¹ Division of Bioinformatics, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, 3052, Australia; Department of Medical Biology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC, 3010, Australia; Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia.
² Division of Bioinformatics, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, 3052, Australia; Department of Medical Biology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC, 3010, Australia.
³ Division of Bioinformatics, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, 3052, Australia; Department of Medical Biology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC, 3010, Australia; South Australian ImmunoGENomics Cancer Institute, The University of Adelaide, SA, Australia.
⁴ Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia.
⁵ Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia; Princess Alexandra Hospital, Woolloongabba, QLD, Australia.
⁶ Nanostring Technologies, Inc, Seattle, WA, USA.
⁷ Pontifícia Universidade Católica do Paraná, PUCPR, Curitiba, Paraná, Brazil.
⁸ Laboratório de Patologia Experimental, PPGCS da PUCPR, Curitiba, Brazil.
⁹ Faculdades Pequeno Príncipe (FPP), Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), R. Silva Jardim, 1632-ÁguaVerde, Curitiba, 80230-020, PR, Brazil.
¹⁰ Westmead Institute for Medical Research, Sydney, Australia.
¹¹ School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Australia.
¹² Critical Care Research Group, The Prince Charles Hospital, Brisbane, Australia; Faculty of Medicine University of Queensland, Brisbane, QLD, Australia; St Andrew's War Memorial Hospital, UnitingCare, Spring Hill, QLD, Australia.
¹³ Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia. Electronic address: arutha.kulasinghe@uq.edu.au.
¹⁴ Division of Bioinformatics, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, 3052, Australia; Department of Medical Biology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC, 3010, Australia; Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia; South Australian ImmunoGENomics Cancer Institute, The University of Adelaide, SA, Australia; Department of Clinical Pathology, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC, 3010, Australia.

Abstract

The utilization of single-cell resolved spatial transcriptomics to delineate immune responses during SARS-CoV-2 infection was able to identify M1 macrophages to have elevated expression of IFI27 in areas of infection.

Publication types

Letter

MeSH terms

COVID-19*
Gene Expression Profiling
Humans
Macrophages
Membrane Proteins
SARS-CoV-2

Substances

IFI27 protein, human
Membrane Proteins