Climate change is predicted to increase atmospheric vapor pressure deficit, exacerbating soil drought, and thus enhancing tree evaporative demand and mortality. Yet, few studies have addressed the longer-term drought acclimation strategy of trees, particularly the importance of morphological vs. hydraulic plasticity. Using a long-term (20 years) irrigation experiment in a natural forest, we investigated the acclimation of Pinus sylvestris morpho-anatomical (stomatal anatomy and crown density) and hydraulic traits (leaf water potential, vulnerability to cavitation Ψ50, hydraulic conductivity Ks, and tree water deficit TWD) to prolonged changes in soil moisture. We found that low water availability reduced twig water potential and increased TWD during the growing season. Still, the trees showed limited adjustments in most branch-level hydraulic traits (Ψ50 and Ks) and needle anatomy. In contrast, trees acclimated to prolonged irrigation by increasing their crown density and, hence, the canopy water demand. This study demonstrates that despite substantial canopy adjustments, P. sylvestris may be vulnerable to extreme droughts because of limited adjustment potential in their hydraulic system. While sparser canopies reduce the water demand, such shifts take decades to occur under chronic water deficits and might not mitigate short-term extreme drought events.
Keywords: Scots pine; acclimation; canopy density; drought; hydraulic conductivity; irrigation; tree water deficit; Ψ50.
© The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology.