Rapid and Self-Administrable Capillary Blood Microsampling Demonstrates Statistical Equivalence with Standard Venous Collections in NMR-Based Lipoprotein Analysis

Jayden Lee Roberts; Luke Whiley; Nicola Gray; Melvin Gay; Philipp Nitschke; Reika Masuda; Elaine Holmes; Jeremy K Nicholson; Julien Wist; Nathan G Lawler

doi:10.1021/acs.analchem.3c05152

Rapid and Self-Administrable Capillary Blood Microsampling Demonstrates Statistical Equivalence with Standard Venous Collections in NMR-Based Lipoprotein Analysis

Anal Chem. 2024 Mar 19;96(11):4505-4513. doi: 10.1021/acs.analchem.3c05152. Epub 2024 Feb 19.

Authors

Jayden Lee Roberts^{1

2}, Luke Whiley^{1

2}, Nicola Gray^{1

2}, Melvin Gay³, Philipp Nitschke^{1

2}, Reika Masuda^{1

2}, Elaine Holmes^{1

2

4}, Jeremy K Nicholson^{1

2

5

6}, Julien Wist^{1

2

7

8}, Nathan G Lawler^{1

2}

Affiliations

¹ Australian National Phenome Centre, Health Futures Institute, Harry Perkins Institute, Murdoch University, 5 Robin Warren Drive, Murdoch, WA 6150, Australia.
² Centre for Computational and Systems Medicine, Health Futures Institute, Harry Perkins Institute, Murdoch University, 5 Robin Warren Drive, Murdoch, WA 6150, Australia.
³ Bruker Pty Ltd., Preston, VIC 3072, Australia.
⁴ Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, South Kensington, London SW7 2AZ, U.K.
⁵ Department of Cardiology, Fiona Stanley Hospital, Medical School, University of Western Australia, Murdoch, WA 6150, Australia.
⁶ Institute of Global Health Innovation, Faculty of Medicine, Imperial College London, Level 1, Faculty Building, South Kensington, London SW7 2NA, U.K.
⁷ Faculty of Medicine, Department of Metabolism, Digestion and Reproduction, Division of Digestive Diseases, Imperial College, London SW7 2AZ, United Kingdom.
⁸ Chemistry Department, Universidad del Valle, Melendez 76001, Cali, Colombia.

Abstract

We investigated plasma and serum blood derivatives from capillary blood microsamples (500 μL, MiniCollect tubes) and corresponding venous blood (10 mL vacutainers). Samples from 20 healthy participants were analyzed by ¹H NMR, and 112 lipoprotein subfraction parameters; 3 supramolecular phospholipid composite (SPC) parameters from SPC₁, SPC₂, and SPC₃ subfractions; 2 N-acetyl signals from α-1-acid glycoprotein (Glyc), GlycA, and GlycB; and 3 calculated parameters, SPC (total), SPC₃/SPC₂, and Glyc (total) were assessed. Using linear regression between capillary and venous collection sites, we explained that agreement (Adj. R² ≥ 0.8, p < 0.001) was witnessed for 86% of plasma parameters (103/120) and 88% of serum parameters (106/120), indicating that capillary lipoprotein, SPC, and Glyc concentrations follow changes in venous concentrations. These results indicate that capillary blood microsamples are suitable for sampling in remote areas and for high-frequency longitudinal sampling of the majority of lipoproteins, SPCs, and Glycs.

MeSH terms

Humans
Lipoproteins*
Magnetic Resonance Spectroscopy
Plasma
Specimen Handling*

Substances

Lipoproteins