Chimeric provirus of bovine leukemia virus/SMAD family member 3 in cattle with enzootic bovine leukosis

Naganori Nao; Tomohiro Okagawa; Naomi Nojiri; Satoru Konnai; Honami Shimakura; Misono Tominaga; Hazuka Yoshida-Furihata; Eri Nishiyama; Takahiro Matsudaira; Naoya Maekawa; Shiro Murata; Masamichi Muramatsu; Kazuhiko Ohashi; Masumichi Saito

doi:10.1007/s00705-024-05970-3

Chimeric provirus of bovine leukemia virus/SMAD family member 3 in cattle with enzootic bovine leukosis

Arch Virol. 2024 Feb 16;169(3):47. doi: 10.1007/s00705-024-05970-3.

Authors

Naganori Nao^{1

2

3}, Tomohiro Okagawa⁴, Naomi Nojiri⁵, Satoru Konnai^{6

7}, Honami Shimakura⁸, Misono Tominaga⁸, Hazuka Yoshida-Furihata⁵, Eri Nishiyama⁹, Takahiro Matsudaira⁹, Naoya Maekawa⁴, Shiro Murata^{4

8}, Masamichi Muramatsu¹⁰, Kazuhiko Ohashi^{4

8}, Masumichi Saito^{11

12}

Affiliations

¹ Division of International Research Promotion, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Japan.
² One Health Research Center, Hokkaido University, Sapporo, Japan.
³ Institute for Vaccine Research and Development (IVReD), Hokkaido University, Sapporo, Japan.
⁴ Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
⁵ Center for Emergency Preparedness and Response, National Institute of Infectious Diseases, Tokyo, Japan.
⁶ Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan. konnai@vetmed.hokudai.ac.jp.
⁷ Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan. konnai@vetmed.hokudai.ac.jp.
⁸ Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
⁹ Biotechnological Research Support Division, FASMAC Co., Ltd, Atsugi, Japan.
¹⁰ Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
¹¹ Center for Emergency Preparedness and Response, National Institute of Infectious Diseases, Tokyo, Japan. saitomas@niid.go.jp.
¹² Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan. saitomas@niid.go.jp.

PMID: 38366081
DOI: 10.1007/s00705-024-05970-3

Abstract

Bovine leukemia virus (BLV) is a member of the family Retroviridae that causes enzootic bovine leukemia (EBL). However, the association between BLV infection and EBL development remains unclear. In this study, we identified a BLV/SMAD3 chimeric provirus within CC2D2A intron 30 in monoclonal expanded malignant cells from a cow with EBL. The chimeric provirus harbored a spliced SMAD3 sequence composed of exons 3-9, encoding the short isoform protein, and the BLV-SMAD3 chimeric transcript was detectable in cattle with EBL. This is the first report of a BLV chimeric provirus that might be involved in EBL tumorigenesis.

MeSH terms

Animals
Cattle
Enzootic Bovine Leukosis*
Female
Leukemia Virus, Bovine* / genetics
Proviruses / genetics

Abstract

MeSH terms

Grants and funding