Method development of multi pesticide residue analysis in country beans collected from Dhaka, Bangladesh, and their dietary risk assessment

Abstract

The aim of the study was to develop a modified QuEChERS method coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of five multi-class pesticides in country beans collected from Dhaka, Bangladesh. Pesticides were extracted using ACN, and to minimize the co-extraction matrix, optimized d-SPE cleanup was done using sorbents (GCB, PSA, and C18). In the calibration range, the method showed excellent linearity with a correlation coefficient of R² ≥ 0.9990 both in solvent- and matrix-matched calibration. For the selected pesticides, average recoveries (at four spiking levels (n = 5) of 10, 20, 100, and 200 µg/kg) of 70-100 % were achieved with relative standard deviations (RSDs) ≤ 9.5 %. The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.3333 to 1.3333 μg/kg and 1.0 to 4.0 μg/kg, respectively. The dietary risk assessment, in terms of hazard quotient (HQ), was calculated to assess consumers' health risks.

Keywords: Azoxystrobin; Buprofezin; Carbofuran; Dimethoate; Health Risk; LC-MS/MS; Pesticide residue; Pymetrozine.