Nanocrystal Structure of Minoxidil: A Safe Stimulator for Hair Growth Factor for C57BL/6 Mice

Ali Moradi; Seyed Morteza Seifati; Majid Darroudi; Shiva Golmohammadzadeh; Mahmood Dehghani Ashkezari

doi:10.2174/0109298673283413240116065844

Nanocrystal Structure of Minoxidil: A Safe Stimulator for Hair Growth Factor for C57BL/6 Mice

Curr Med Chem. 2024 Feb 15. doi: 10.2174/0109298673283413240116065844. Online ahead of print.

Authors

Ali Moradi¹, Seyed Morteza Seifati¹, Majid Darroudi², Shiva Golmohammadzadeh^{3

4}, Mahmood Dehghani Ashkezari¹

Affiliations

¹ Department of Biology, Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran.
² Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
³ Department of Pharmaceutics, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
⁴ Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.

PMID: 38362689
DOI: 10.2174/0109298673283413240116065844

Abstract

Background: Commercial Minoxidil (MXD) is commonly used as a vasodilator agent of hair follicles for providing direct dermal papilla cell proliferation and consequently enhancing the rate of hair growth.

Objective: The current study attempted to improve the bioactivity and water solubility of MXD by producing nanocrystal structures and investigating the obtained hair growthstimulating activity on C57BL/6 mice.

Method: The MXD nanoparticles (MXD-NPs) were prepared through a bead mill and ultrasonic process and characterized by DLS, XRD, UV-Vis, FTIR, FESEM, TEM, and Zeta-potential techniques.

Result: The cytotoxicity of MXD-NPs was studied on human dermal fibroblast (HDF) by MTT assay. Lastly, we analyzed the comparative hair growth inductive activity of certain MXD-NPs concentrations on C57BL/6 mice. The stabled MXD-NPs (-46 mV, 21.9 nm) caused a significant increase in the hair growth rate of C57BL/6 mice by running a safe site-specific delivery mechanism on the targeted pilosebaceous follicles when compared to MXD.

Conclusion: The MXD-NPs-receiving mice exhibited a greater rate of anagen/telogen follicular when compared with MXD-treated types, which verified the improvement of their hair re-growing and follicular-stimulative activities. Therefore, these outcomes confirmed the potential of MXD-NPs for substituting its commercial solution format as a safe and efficient iso-formulation structure.

Keywords: C57BL/6 mice;; Cytotoxicity;; Hair growth stimulating activity; Human dermal fibroblast (HDF);; Hydrothermal;; Minoxidil nanoparticles (MXD-NPs);.