Caspase-1 location in cells has been studied with fluorochrome-labeled inhibitors of caspase-1 (FLICA reagents). We report that FLICA reagents have limited cell-membrane permeability. This impacts experimental design as cells with intact membranes, including caspase-1 knockout cells, are not appropriate controls for cells with inflammasome-induced gasdermin D membrane pores.
Keywords: Caspase inhibitor; Caspase-1; FLICA; Gasdermin D; Inflammasome.
© 2024 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.