A gene-encoded aldehyde tag repurposed from RiPP cyclophane-forming pathway

Suze Ma; Heng Chen; Shuxun Liu; Xuedong Huang; Tianlu Mo; Wan-Qiu Liu; Wei Zhang; Wei Ding; Qi Zhang

doi:10.1016/j.bmcl.2024.129653

A gene-encoded aldehyde tag repurposed from RiPP cyclophane-forming pathway

Bioorg Med Chem Lett. 2024 Mar 15:101:129653. doi: 10.1016/j.bmcl.2024.129653. Epub 2024 Feb 13.

Authors

Suze Ma¹, Heng Chen², Shuxun Liu¹, Xuedong Huang¹, Tianlu Mo³, Wan-Qiu Liu⁴, Wei Zhang⁵, Wei Ding⁶, Qi Zhang⁷

Affiliations

¹ Department of Chemistry, Fudan University, Shanghai 200433, China.
² Department of Chemistry, Fudan University, Shanghai 200433, China; State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
³ School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
⁴ School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.
⁵ Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.
⁶ State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
⁷ Department of Chemistry, Fudan University, Shanghai 200433, China. Electronic address: qizhang@sioc.ac.cn.

PMID: 38360420
DOI: 10.1016/j.bmcl.2024.129653

Abstract

Gene-encoded aldehyde tag technology has been widely utilized in protein bioorthogonal chemistry and biotechnological application. Herein, we report utilization of the promiscuous rSAM cyclophane synthase SjiB involved in triceptide biosynthesis as a dedicated and highly efficient formylglycine synthase. The new aldehyde tag sequence in this system, YQSSI, is biosynthetically orthogonal to the known aldehyde tag (C/S)x(P/A)xR. The potential use of SjiB/YQSSI aldehyde tag system was further validated in fluorescent labelling of model proteins.

MeSH terms

Aldehydes*
Cyclophanes*
Proteins

Substances

Cyclophanes
Aldehydes
Proteins