Mechanical strain regulates osteogenesis via Antxr1/LncRNA H19/Wnt/β-catenin axis

Lifei Liu; Jianmin Guo; Xiaoyang Tong; Miao Zhang; Xi Chen; Mei Huang; Chenyu Zhu; Samuel Bennett; Jiake Xu; Jun Zou

doi:10.1002/jcp.31214

Mechanical strain regulates osteogenesis via Antxr1/LncRNA H19/Wnt/β-catenin axis

J Cell Physiol. 2024 Feb 15. doi: 10.1002/jcp.31214. Online ahead of print.

Authors

Lifei Liu^{1

2}, Jianmin Guo^{1

3}, Xiaoyang Tong^{1

4}, Miao Zhang^{1

5}, Xi Chen⁶, Mei Huang¹, Chenyu Zhu¹, Samuel Bennett⁷, Jiake Xu^{7

8}, Jun Zou¹

Affiliations

¹ School of Exercise and Health, Shanghai University of Sport, Shanghai, China.
² Department of Rehabilitation, The People's Hospital of Liaoning Province, Shenyang, China.
³ Department of Biomedical Engineering, Southern University of Science and Technology, Guangzhou, China.
⁴ College of Physical Education, Qingdao University of Science and Technology, Qingdao, China.
⁵ College of Physical Education, Yanshan University, Qinhuangdao, China.
⁶ School of Sports Science, Wenzhou Medical University, Wenzhou, China.
⁷ School of Biomedical Sciences, University of Western Australia, Perth, Western Australia, Australia.
⁸ Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

PMID: 38358001
DOI: 10.1002/jcp.31214

Abstract

Alleviating bone loss is an essential way to prevent osteoporotic fractures. Proper exercise improves bone density without the side effects of long-term medications, but the mechanism is unclear. Our study explored the role of Antxr1/LncRNA H19/Wnt/β-catenin axis in the process of exercise-mediated alleviation of bone loss. Here we discovered that moderate-intensity treadmill exercise alleviates bone loss caused by ovariectomy and ameliorates bone strength accompanied by an increased lncRNA H19 expression. Concomitantly, Antxr1, a mechanosensitive protein was found downregulated by exercise but upregulated by ovariectomy. Interestingly, knockdown expression of Antxr1 increased lncRNA H19 expression and Wnt/β-catenin signaling pathway in bone marrow mesenchymal stem cells, whereas overexpression of Antxr1 decreased lncRNA H19 expression and Wnt/β-catenin signaling pathway. Hence, our study demonstrates the regulation of Antxr1/LncRNA H19/Wnt/β-catenin axis in the process of mechanical strain-induced osteogenic differentiation, which provides further mechanistic insight into the role of mechanical regulation in bone metabolism.

Keywords: Antxr1; Wnt/β-catenin; exercise; lncRNA H19; mechanical strain; osteoporosis.

Abstract

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