Insights into metabolic characteristics and biological activity changes in Zangju (Citrus reticulata cv. Manau Gan) peel at different maturity stages through UPLC-MS/MS-based metabolomics

Peng Wang; Haifan Wang; Yang Xiao; Jialiang Zou; Hongping Chen; Lin Chen; Fu Wang; Yuan Hu; Youping Liu

doi:10.1016/j.fochx.2024.101197

Insights into metabolic characteristics and biological activity changes in Zangju (Citrus reticulata cv. Manau Gan) peel at different maturity stages through UPLC-MS/MS-based metabolomics

Food Chem X. 2024 Feb 5:21:101197. doi: 10.1016/j.fochx.2024.101197. eCollection 2024 Mar 30.

Authors

Peng Wang^{1

2}, Haifan Wang^{1

2}, Yang Xiao¹, Jialiang Zou^{1

2}, Hongping Chen^{1

2}, Lin Chen^{1

2}, Fu Wang^{1

2}, Yuan Hu^{1

2}, Youping Liu^{1

2}

Affiliations

¹ Department of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
² State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu 611137, China.

Abstract

In this study, comprehensive and systematic nontargeted metabolomics analysis was performed with the metabolites of Zangju peel (Citrus reticulata cv. Manau Gan, CRZP, which has been cultivated for over 400 years in Derong County, China.) at four different mature stages. A total of 1878 metabolites were identified, among which flavonoids were the most abundant (62.04 %), and identified 62 key differential metabolites significantly affected by maturity. Based on biological activity measurements, CRZP showed better antioxidant activity, lipase inhibition ability, inhibition of adipogenic differentiation in 3TT-L1 cells and promotion of lipid metabolism, with the biological activity of CRZP at different maturity stages being associated with key differential metabolite. Thus, CRZP is natural antioxidants and possess anti-obesity potential, and industrial production needs to consider the Maturity stage of its collection.

Keywords: Biological activity; Citrus reticulata cv. Manau Gan; Differentially accumulated metabolites; Maturity stage; Nontargeted metabolomics.