Fabrication of Rosuvastatin-Incorporated Polycaprolactone -Gelatin Scaffold for Bone Repair: A Preliminary In Vitro Study

Maliheh Gharibshahian; Morteza Alizadeh; Mohammad Kamalabadi Farahani; Majid Salehi

doi:10.22074/cellj.2023.2009047.1391

Fabrication of Rosuvastatin-Incorporated Polycaprolactone -Gelatin Scaffold for Bone Repair: A Preliminary In Vitro Study

Cell J. 2024 Jan 31;26(1):70-80. doi: 10.22074/cellj.2023.2009047.1391.

Authors

Maliheh Gharibshahian¹, Morteza Alizadeh², Mohammad Kamalabadi Farahani², Majid Salehi^{3

4

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Affiliations

¹ Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
² Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
³ Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran. Email: Salehi.m@shmu.ac.ir.
⁴ Tissue Engineering and Stem Cells Research Centre, Shahroud University of Medical Sciences, Shahroud, Iran.
⁵ Sexual Health and Fertility Research Centre, Shahroud University of Medical Sciences, Shahroud, Iran.

Abstract

Objective: Rosuvastatin (RSV) is a hydrophilic, effective statin with a long half-life that stimulates bone regeneration. The present study aims to develop a new scaffold and controlled release system for RSV with favourable properties for bone tissue engineering (BTE).

Materials and methods: In this experimental study, high porous polycaprolactone (PCL)-gelatin scaffolds that contained different concentrations of RSV (0 mg/10 ml, 0.1 mg/10 ml, 0.5 mg/10 ml, 2.5 mg/10 ml, 12.5 mg/10 ml, and 62.5 mg/10 ml) were fabricated by the thermally-induced phase separation (TIPS) method. Mechanical and biological properties of the scaffolds were evaluated by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), compressive strength, porosity, MTT, alkaline phosphatase (ALP) activity, water contact angle, degradation rate, pH alteration, blood clotting index (BCI), and hemocompatibility.

Results: SEM analysis confirmed that the porous structure of the scaffolds contained interconnected pores. FTIR results showed that the RSV structure was maintained during the scaffold's fabrication. RSV (up to 62.5 mg/10 ml) increased compressive strength (16.342 ± 1.79 MPa), wettability (70.2), and degradation rate of the scaffolds. Scaffolds that contained 2.5 mg/10 ml RSV had the best effect on the human umbilical cord mesenchymal stem cell (HUC-MSCs) survival, hemocompatibility, and BCI. As a sustained release system, only 31.68 ± 0.1% of RSV was released from the PCL-Gelatin-2.5 mg/10 ml RSV scaffold over 30 days. In addition, the results of ALP activity showed that RSV increased the osteogenic differentiation potential of the scaffolds.

Conclusion: PCL-Gelatin-2.5 mg/10 ml RSV scaffolds have favorable mechanical, physical, and osteogenic properties for bone tissue and provide a favorable release system for RSV. They can mentioned as a a promising strategy for bone regeneration that should be further assessed in animals and clinical studies.

Keywords: Bone; Gelatin; Polycaprolactone; Regeneration; Rosuvastatin.