Isolation and In Vitro Propagation of Human Spermatogonial Stem Cells (SSCs)

Janmejay Hingu; Guillermo Galdon; Nicholas A Deebel; Hooman Sadri-Ardekani

doi:10.1007/978-1-0716-3698-5_3

Isolation and In Vitro Propagation of Human Spermatogonial Stem Cells (SSCs)

Methods Mol Biol. 2024:2770:27-36. doi: 10.1007/978-1-0716-3698-5_3.

Authors

Janmejay Hingu^{1

2}, Guillermo Galdon², Nicholas A Deebel^{1

2}, Hooman Sadri-Ardekani^{3

4}

Affiliations

¹ Department of Urology, Wake Forest University School of Medicine, Winston-Salem, NC, USA.
² Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, USA.
³ Department of Urology, Wake Forest University School of Medicine, Winston-Salem, NC, USA. hsadri@wakehealth.edu.
⁴ Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, USA. hsadri@wakehealth.edu.

PMID: 38351444
DOI: 10.1007/978-1-0716-3698-5_3

Abstract

Preservation of human spermatogonial stem cells (SSCs) may be suitable for young male patients at risk of male infertility due to various causes, such as gonadotoxic treatment or genetic diseases. With optimal cryopreservation, cell viability can be retained to reestablish spermatogenesis in the future through autologous transplantation or in vitro differentiation of SSCs. This protocol outlines techniques to optimize the SSCs isolation and in vitro culture. With particular emphasis on the microscopic characteristics encountered, this protocol outlines a broader approach to processing tissues with varying morphologies among patients.

Keywords: Isolation; Male infertility; Propagation; Spermatogonial stem cell (SSC).

MeSH terms

Adult Germline Stem Cells*
Cryopreservation / methods
Humans
Infertility, Male*
Male
Spermatogenesis
Spermatogonia
Testis