Adhesion Inhibition Assay for Helicobacter pylori to Mucin by Lactobacillus

Keita Nishiyama; Takao Mukai

doi:10.1007/978-1-0716-3670-1_30

Adhesion Inhibition Assay for Helicobacter pylori to Mucin by Lactobacillus

Methods Mol Biol. 2024:2763:353-358. doi: 10.1007/978-1-0716-3670-1_30.

Authors

Keita Nishiyama^{1

2}, Takao Mukai³

Affiliations

¹ Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. keita.nishiyama.a6@tohoku.ac.jp.
² Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. keita.nishiyama.a6@tohoku.ac.jp.
³ Department of Animal Science, School of Veterinary Medicine, Kitasato University, Aomori, Japan.

PMID: 38347425
DOI: 10.1007/978-1-0716-3670-1_30

Abstract

The ability of Lactobacillus to adhere to mucin is a parameter for evaluating the effectiveness of probiotics. In particular, a competitive inhibition assay of pathogenic bacteria using mucin-adherent lactobacilli is useful for identifying Lactobacillus strains capable of preventing mucus from being colonized by pathogens. Here, we describe an adhesion inhibition assay method for Helicobacter pylori to porcine gastric mucin by Limosilactobacillus reuteri.

Keywords: Adhesion; Helicobacter; Lactobacillus; Mucin; Probiotics.

MeSH terms

Animals
Bacterial Adhesion / physiology
Helicobacter Infections*
Helicobacter pylori*
Lactobacillus / physiology
Mucins
Probiotics*
Swine

Substances

Mucins