MALDI-TOF MS/MS Analysis of Permethylated O-Glycan Alditols Released from Mucins

Yukinobu Goso; Makoto Kurihara

doi:10.1007/978-1-0716-3670-1_17

MALDI-TOF MS/MS Analysis of Permethylated O-Glycan Alditols Released from Mucins

Methods Mol Biol. 2024:2763:201-208. doi: 10.1007/978-1-0716-3670-1_17.

Authors

Yukinobu Goso¹, Makoto Kurihara¹

Affiliation

¹ Department of Applied Bioscience, Kanagawa Institute of Technology, Atsugi, Japan.

PMID: 38347412
DOI: 10.1007/978-1-0716-3670-1_17

Abstract

Mucin glycans are associated with the function of mucin in maintaining mucosal homeostasis. Therefore, the glycomic analysis of mucins is crucial. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is one of the most suitable methods for the glycomic analysis of mucin O-glycans. In this chapter, we describe methods for analyzing permethylated O-glycan alditols released from mucins by MALDI-TOF MS and MALDI-TOF tandem mass spectrometry (MALDI-TOF MS/MS).

Keywords: Glycomics; MALDI-TOF MS; Mass spectrometry; Mucins; O-Glycan alditols; Permethylation.

MeSH terms

Mucins* / chemistry
Polysaccharides / chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Tandem Mass Spectrometry*

Substances

Mucins
Polysaccharides