Preparation of Jellyfish Mucin

Kiminori Ushida; Hiroshi Inui; Takuma Kaneko; Shinra Tanaka; Anri Mochizuki; Shiori Kaise; Minami Sugiyama

doi:10.1007/978-1-0716-3670-1_1

Preparation of Jellyfish Mucin

Methods Mol Biol. 2024:2763:3-36. doi: 10.1007/978-1-0716-3670-1_1.

Authors

Kiminori Ushida¹, Hiroshi Inui², Takuma Kaneko², Shinra Tanaka², Anri Mochizuki², Shiori Kaise², Minami Sugiyama²

Affiliations

¹ Department of Chemistry, School of Science, Kitasato University, Sagamihara, Japan. ushidak@kitasato-u.ac.jp.
² Department of Chemistry, School of Science, Kitasato University, Sagamihara, Japan.

PMID: 38347396
DOI: 10.1007/978-1-0716-3670-1_1

Abstract

A mucin-type glycoprotein extracted from various species of jellyfish (JF) is named qniumucin (Q-mucin). Compared with general mucins, most of which are from mammals including humans, Q-mucin can be collected on a relatively large scale with high yield. Owing to its simple structure with low heterogeneity, Q-mucin has a potential to be developed into material mucins which opens various applications valuable to humans. On the basis of our present knowledge, here, we describe our protocol for the extraction of Q-mucin, which can be extracted from any JF species worldwide. Experimental protocols to identify the structure of Q-mucin are also introduced.

Keywords: Amino acid composition analysis; Anion exchange chromatography; Extracellular matrix; Glycoform analysis; Jellyfish mucin; Mass spectrometry; Mucin; Mucin extraction; Mucin purification.

MeSH terms

Animals
Humans
Mammals
Mucins* / chemistry
Scyphozoa* / chemistry

Substances

Mucins