Purpose: This study aims to decellularized caprine pericardium tissue with varied non-ionic surfactant and anionic detergent concentrations.
Methods: Protocol A consists of 1%, 0.5%, and 0.25% (w/v) sodium dodecyl sulphate (SDS). Protocol B uses 1%, 0.5%, and 0.25% (w/v) Triton X-100. Protocol C comprised 0.5% SDS + 0.5% Triton X-100, 0.5% + 0.25%, and 0.25% SDS + 0.5% Triton X-100.
Results: Protocol B left a few countable cells in the pericardium tissue, but treatments A and C removed all cells. DNA quantification also demonstrated that protocol B had the most leftover DNA after decellularization. The pericardium tissue treated with an equal combination of anionic detergent and non-ionic surfactant preserves the matrix. However, changing the anionic detergent-non-ionic surfactant ratio disrupted the microstructure. Protocol A decreased pericardium tissue secant modulus (p < 0.05). Protocol B-treated pericardium tissue matched native tissue secant modulus and ultimate tensile stress. Protocol C strengthened pericardium tissue.
Conclusion: The intact extracellular matrix and biomechanical properties like native tissues require optimal chemical doses and combinations.
Keywords: Acellularized scaffolds; Detergents; Extracellular matrix; Surfactant; Xenograft.
© 2024. The Author(s) under exclusive licence to Biomedical Engineering Society.