Effects of incubation temperature on the upper thermal tolerance of the imperiled longfin smelt (Spirinchus thaleichthys)

Yuzo R Yanagitsuru; Florian Mauduit; Alexis J Lundquist; Levi S Lewis; James A Hobbs; Tien-Chieh Hung; Richard E Connon; Nann A Fangue

doi:10.1093/conphys/coae004

Effects of incubation temperature on the upper thermal tolerance of the imperiled longfin smelt (Spirinchus thaleichthys)

Conserv Physiol. 2024 Feb 10;12(1):coae004. doi: 10.1093/conphys/coae004. eCollection 2024.

Authors

Yuzo R Yanagitsuru¹, Florian Mauduit^{1

2}, Alexis J Lundquist¹, Levi S Lewis¹, James A Hobbs^{1

3}, Tien-Chieh Hung⁴, Richard E Connon², Nann A Fangue¹

Affiliations

¹ Department of Wildlife, Fish, and Conservation Biology, University of California, Davis, One Shields Avenue, Davis, CA, Yolo County, 95616, USA.
² Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, One Shields Avenue, Davis, CA, Yolo County, 95616, USA.
³ California Department of Fish and Wildlife, Bay-Delta IEP, 2109 Arch Airport Rd,Stockton, CA, San Joaquin County, 95206, USA.
⁴ Fish Conservation and Culture Laboratory, Department of Biological and Agricultural Engineering, University of California, Davis, 17501 Byron Hwy, Byron, CA, Contra Costa County, 94514, USA.

Abstract

Upper thermal limits in many fish species are limited, in part, by the heart's ability to meet increased oxygen demand during high temperatures. Cardiac plasticity induced by developmental temperatures can therefore influence thermal tolerance. Here, we determined how incubation temperatures during the embryonic stage influence cardiac performance across temperatures during the sensitive larval stage of the imperiled longfin smelt. We transposed a cardiac assay for larger fish to newly hatched larvae that were incubated at 9°C, 12°C or 15°C. We measured heart rate over increases in temperature to identify the Arrhenius breakpoint temperature (T_AB), a proxy for thermal optimum and two upper thermal limit metrics: temperature when heart rate is maximized (T_peak) and when cardiac arrhythmia occurs (T_Arr). Higher incubation temperatures increased T_AB, T_peak and T_Arr, but high individual variation in all three metrics resulted in great overlap of individuals at T_AB, T_peak and T_Arr across temperatures. We found that the temperatures at which 10% of individuals reached T_peak or T_Arr and temperatures at which number of individuals at T_AB relative to T_peak (ΔN(T_AB,T_peak)) was maximal, correlated more closely with upper thermal limits and thermal optima inferred from previous studies, compared to the mean values of the three cardiac metrics of the present study. Higher incubation temperatures increased the 10% T_peak and T_Arr thresholds but maximum ΔN(T_AB,T_peak) largely remained the same, suggesting that incubation temperatures modulate upper thermal limits but not T_opt for a group of larvae. Overall, by measuring cardiac performance across temperatures, we defined upper thermal limits (10% thresholds; T_peak, 14.4-17.5°C; T_Arr, 16.9-20.2°C) and optima (ΔN(T_AB,T_peak), 12.4-14.4°C) that can guide conservation strategies for longfin smelt and demonstrated the potential of this cardiac assay for informing conservation plans for the early life stages of fish.

Keywords: Arrhenius breakpoint; San Francisco Estuary; cardiac performance; climate change; heart rate; larvae; thermal performance.