Comparative modeling of fluorescence and P700 induction kinetics for alga Scenedesmus sp. obliques and cyanobacterium Synechocystis sp. PCC 6803. Role of state 2-state 1 transitions and redox state of plastoquinone pool

N E Belyaeva; A A Bulychev; K E Klementiev; V Z Paschenko; G Yu Riznichenko; A B Rubin

doi:10.1007/s12013-024-01224-w

Comparative modeling of fluorescence and P700 induction kinetics for alga Scenedesmus sp. obliques and cyanobacterium Synechocystis sp. PCC 6803. Role of state 2-state 1 transitions and redox state of plastoquinone pool

Cell Biochem Biophys. 2024 Feb 10. doi: 10.1007/s12013-024-01224-w. Online ahead of print.

Authors

N E Belyaeva¹, A A Bulychev², K E Klementiev^{2

3}, V Z Paschenko², G Yu Riznichenko², A B Rubin²

Affiliations

¹ Biological Faculty, Moscow State University, Moscow, 119234, Russia. natalmurav@yandex.ru.
² Biological Faculty, Moscow State University, Moscow, 119234, Russia.
³ Biological Faculty, Shenzhen MSU-BIT University, Shenzhen, 518172, China.

PMID: 38340281
DOI: 10.1007/s12013-024-01224-w

Abstract

The model of thylakoid membrane system (T-M model) (Belyaeva et al. Photosynth Res 2019, 140:1-19) has been improved in order to analyze the induction data for dark-adapted samples of algal (Scenedesmus obliques) and cyanobacterial (Synechocystis sp. PCC 6803) cells. The fluorescence induction (FI) curves of Scenedesmus were measured at light exposures of 5 min, while FI and P700 redox transformations of Synechocystis were recorded in parallel for 100 s intervals. Kinetic data comprising the OJIP-SMT fluorescence induction and OABCDEF P700⁺ absorbance changes were used to study the processes underlying state transitions qT_2→1 and qT_1→2 associated with the increase/decrease in Chl fluorescence emission. A formula with the Hill kinetics (Ebenhöh et al. Philos Trans R Soc B 2014, 369:20130223) was introduced into the T-M model, with a new variable to imitate the flexible size of antenna Ant_M(t) associated with PSII. Simulations revealed that the light-harvesting capacity of PSII increases with a corresponding decrease for that of PSI upon the qT_2→1 transition induced by plastoquinone (PQ) pool oxidation. The complete T-M model fittings were attained on Scenedesmus or Synechocystis fast waves OJIPS of FI, while SMT wave of FI was reproduced at intervals shorter than 5 min. Also the fast P700 redox transitions (OABC) for Synechocystis were fitted exactly. Reasonable sets of algal and cyanobacterial electron/proton transfer (ET/PT) parameters were found. In the case of Scenedesmus, ET/PT traits remained the same irrespective of modeling with or without qT_2→1 transitions. Simulations indicated a high extent (20%) of the PQ pool reduction under dark conditions in Synechocystis compared to 2% in Scenedesmus.

Keywords: Chlorophyll a fluorescence induction; Dissipative energy losses; Electron transfer; Photosynthetic induction; Photosystems I and II; Proton transfer.