Generation of induced pluripotent stem cells (ZZUCSBi001-A) from skin fibroblasts of a healthy donor

Stem Cell Res. 2024 Apr:76:103328. doi: 10.1016/j.scr.2024.103328. Epub 2024 Feb 5.

Abstract

Fibroblasts were extracted from the scalp of a healthy 55-year-old male and subsequently transformed into pluripotent stem cells by introducing episomal plasmids harboring essential reprogramming factors. These induced pluripotent stem cells exhibited a normal karyotype and demonstrated the capacity to differentiate into all three germ layers, as confirmed through teratoma assays. This specific cell line serves as a valuable reference for comparative investigations alongside other induced pluripotent stem cell lines generated from somatic cells of patients afflicted by genetic neurodegenerative disorders.

MeSH terms

  • Cell Differentiation
  • Cell Line
  • Cellular Reprogramming
  • Fibroblasts / metabolism
  • Humans
  • Induced Pluripotent Stem Cells* / metabolism
  • Male
  • Middle Aged
  • Plasmids
  • Teratoma* / metabolism